Mouse Cystatin C PicoKine® Quick ELISA Kit

Mouse Cystatin C/CST3 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse Cst3 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure. Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In mouses, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes) . Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 437 434 446 503 455 28.06 6.1% Sample 2 2310 2387 2135 2376 2302 100.81 4.3% Sample 3 9815 8364 8977 9330 9121 528.93 5.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 437 434 446 503 455 28.06 6.1% Sample 1 437 434 446 503 455 28.06 6.1% Sample 2 2310 2387 2135 2376 2302 100.81 4.3% Sample 2 2310 2387 2135 2376 2302 100.81 4.3% Sample 3 9815 8364 8977 9330 9121 528.93 5.7% Sample 3 9815 8364 8977 9330 9121 528.93 5.7%

Synonyms

Cystatin-C; Cystatin-3; Cst3

Gene Name

CST3

Gene ID

13010

UniProt

P21460

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-A140

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 437 2310 9815 464 2268 10497 Standard deviation 27.96 97.02 706.68 34.8 106.59 871.25 CV (%) 6.4% 4.2% 7.2% 7.5% 4.7% 8.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 437 2310 9815 464 2268 10497 Standard deviation 27.96 97.02 706.68 34.8 106.59 871.25 CV (%) 6.4% 4.2% 7.2% 7.5% 4.7% 8.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 437 2310 9815 464 2268 10497 Mean (pg/ml) 437 2310 9815 464 2268 10497 Standard deviation 27.96 97.02 706.68 34.8 106.59 871.25 Standard deviation 27.96 97.02 706.68 34.8 106.59 871.25 CV (%) 6.4% 4.2% 7.2% 7.5% 4.7% 8.3% CV (%) 6.4% 4.2% 7.2% 7.5% 4.7% 8.3%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

15531 MW

Specificity

Natural and recombinant mouse Cystatin C

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Cystatin-C

Protein Name

Cystatin-C

Sample Data

Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.040 0.148 0.294 0.544 0.802 1.134 1.695 1.962 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.040 0.148 0.294 0.544 0.802 1.134 1.695 1.962 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.040 0.148 0.294 0.544 0.802 1.134 1.695 1.962 O.D. 0.040 0.148 0.294 0.544 0.802 1.134 1.695 1.962

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.