Human CCN1 PicoKine® Quick ELISA Kit

Human CYR61/CCN1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CCN1 in cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cysteine-rich angiogenic inducer 61 (CYR61) or CCN family member 1 (CCN1), is a matricellular protein that in humans is encoded by the CYR61 gene. This gene is mapped to 1p22.3. CYR61 is capable of regulating a broad range of cellular activities, including cell adhesion, migration, proliferation, differentiation, apoptosis, and senescence through interaction with cell surface integrin receptors and heparan sulfate proteoglycans. During embryonic development, CYR61 is critical for cardiac septal morphogenesis, blood vessel formation in placenta, and vascular integrity. In adulthood CYR61 plays important roles in inflammation and tissue repair, and is associated with diseases related to chronic inflammation, including rheumatoid arthritis, atherosclerosis, diabetes-related nephropathy and retinopathy, and many different forms of cancers.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 61 64 63 58 61 2.29 3.7% Sample 2 248 273 263 255 259 9.31 3.5% Sample 3 814 907 783 869 843 47.99 5.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 61 64 63 58 61 2.29 3.7% Sample 1 61 64 63 58 61 2.29 3.7% Sample 2 248 273 263 255 259 9.31 3.5% Sample 2 248 273 263 255 259 9.31 3.5% Sample 3 814 907 783 869 843 47.99 5.6% Sample 3 814 907 783 869 843 47.99 5.6%

Synonyms

Protein CYR61; CCN family member 1; Cysteine-rich angiogenic inducer 61; Insulin-like growth factor-binding protein 10; IBP-10; IGF-binding protein 10; IGFBP-10; Protein GIG1; CYR61; CCN1, GIG1, IGFBP10

Gene Name

CYR61

Gene ID

3491

UniProt

O00622

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: A22-D381

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA), saliva, urine and human milk.

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 61 248 814 59 242 806 Standard deviation 2.86 16.12 55.35 3.3 17.66 64.48 CV (%) 4.7% 6.5% 6.8% 5.6% 7.3% 8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 61 248 814 59 242 806 Standard deviation 2.86 16.12 55.35 3.3 17.66 64.48 CV (%) 4.7% 6.5% 6.8% 5.6% 7.3% 8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 61 248 814 59 242 806 Mean (pg/ml) 61 248 814 59 242 806 Standard deviation 2.86 16.12 55.35 3.3 17.66 64.48 Standard deviation 2.86 16.12 55.35 3.3 17.66 64.48 CV (%) 4.7% 6.5% 6.8% 5.6% 7.3% 8% CV (%) 4.7% 6.5% 6.8% 5.6% 7.3% 8%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

42027 MW

Specificity

Natural and recombinant human CYR61

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.037 0.094 0.152 0.258 0.513 0.811 1.520 2.034 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.037 0.094 0.152 0.258 0.513 0.811 1.520 2.034 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.037 0.094 0.152 0.258 0.513 0.811 1.520 2.034 O.D. 0.037 0.094 0.152 0.258 0.513 0.811 1.520 2.034

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.