Human SMAC PicoKine® Quick ELISA Kit

Human Diablo/SMAC PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human DIABLO in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Diablo homolog, mitochondrial, also known as second mitochondria-derived activator of caspases (SMAC), is a protein that in humans is encoded by the DIABLO gene (IAP binding protein with low pI) . It is mapped to 12q24.31. DIABLO can bind mammalian IAP homolog A (MIHA, or API3) and can also interact with MIHB, MIHC, and OpIAP, the baculoviral IAP. This gene encodes an inhibitor of apoptosis protein (IAP) -binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and allows activation of caspases by binding to inhibitor of apoptosis proteins. Over expression of the encoded protein sensitizes tumor cells to apoptosis. A mutation in this gene is associated with young-adult onset of nonsyndromic deafness-64.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 54 54 55 55 54 0.5 0.9% Sample 2 221 218 222 235 224 6.51 2.9% Sample 3 865 888 885 882 880 8.91 1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 54 54 55 55 54 0.5 0.9% Sample 1 54 54 55 55 54 0.5 0.9% Sample 2 221 218 222 235 224 6.51 2.9% Sample 2 221 218 222 235 224 6.51 2.9% Sample 3 865 888 885 882 880 8.91 1% Sample 3 865 888 885 882 880 8.91 1%

Synonyms

Diablo homolog, mitochondrial; IAP-binding protein with low pI; Second mitochondria-derived activator of caspase; Smac; DIABLO; SMAC

Gene Name

DIABLO

Gene ID

56616

UniProt

Q9NR28

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A56-D239

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 54 221 865 53 229 932 Standard deviation 4.05 17.01 38.83 4.61 20.15 48.46 CV (%) 7.5% 7.7% 4.2% 8.7% 8.8% 5.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 54 221 865 53 229 932 Standard deviation 4.05 17.01 38.83 4.61 20.15 48.46 CV (%) 7.5% 7.7% 4.2% 8.7% 8.8% 5.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 54 221 865 53 229 932 Mean (pg/ml) 54 221 865 53 229 932 Standard deviation 4.05 17.01 38.83 4.61 20.15 48.46 Standard deviation 4.05 17.01 38.83 4.61 20.15 48.46 CV (%) 7.5% 7.7% 4.2% 8.7% 8.8% 5.2% CV (%) 7.5% 7.7% 4.2% 8.7% 8.8% 5.2%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

27131 MW

Specificity

Natural and recombinant human Diablo

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Diablo homolog, mitochondrial

Protein Name

Diablo homolog, mitochondrial

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.013 0.072 0.103 0.196 0.371 0.725 1.340 2.021 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.013 0.072 0.103 0.196 0.371 0.725 1.340 2.021 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.013 0.072 0.103 0.196 0.371 0.725 1.340 2.021 O.D. 0.013 0.072 0.103 0.196 0.371 0.725 1.340 2.021

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.