Mouse CXCL13 PicoKine® Quick ELISA Kit

Mouse CXCL13 / BLC / BCA1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse Cxcl13 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

C-X-C motif chemokine 13 (CXCL13), also known as B lymphocyte chemoattractant (BLC), is a protein that in humans is encoded by theCXCL13 gene. CXCL13 is a small cytokine belonging to the CXC chemokine family, and it was located on sequence maps to 4q21, near the genes encoding most CXC chemokines. In T-lymphocytes, CXCL13 expression is thought to reflect a germinal center origin of the T-cell. Hence, expression of CXCL13 in T-cell lymphomas, such as Angioimmunoblastic T-cell Lymphoma, is thought to reflect a germinal center origin of the neoplastic T-cells.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 34 32 29 31 1.8 5.8% Sample 2 182 204 199 194 194 8.16 4.2% Sample 3 491 507 522 441 490 30.47 6.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 34 32 29 31 1.8 5.8% Sample 1 31 34 32 29 31 1.8 5.8% Sample 2 182 204 199 194 194 8.16 4.2% Sample 2 182 204 199 194 194 8.16 4.2% Sample 3 491 507 522 441 490 30.47 6.2% Sample 3 491 507 522 441 490 30.47 6.2%

Synonyms

C-X-C motif chemokine 13; B lymphocyte chemoattractant; CXC chemokine BLC; Small-inducible cytokine B13; Cxcl13; Blc, Scyb13

Gene Name

CXCL13

Gene ID

55985

UniProt

O55038

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: I22-A109

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 182 491 28 184 451 Standard deviation 1.39 8 31.42 1.4 11.22 33.82 CV (%) 4.5% 4.4% 6.4% 5% 6.1% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 182 491 28 184 451 Standard deviation 1.39 8 31.42 1.4 11.22 33.82 CV (%) 4.5% 4.4% 6.4% 5% 6.1% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 31 182 491 28 184 451 Mean (pg/ml) 31 182 491 28 184 451 Standard deviation 1.39 8 31.42 1.4 11.22 33.82 Standard deviation 1.39 8 31.42 1.4 11.22 33.82 CV (%) 4.5% 4.4% 6.4% 5% 6.1% 7.5% CV (%) 4.5% 4.4% 6.4% 5% 6.1% 7.5%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

11927 MW

Specificity

Natural and recombinant mouse CXCL13

Anticoagulant

Heparin or EDTA

Gene Name Synonym

C-X-C motif chemokine 13

Protein Name

C-X-C motif chemokine 13

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.029 0.127 0.209 0.392 0.731 1.471 1.841 2.033 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.029 0.127 0.209 0.392 0.731 1.471 1.841 2.033 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.029 0.127 0.209 0.392 0.731 1.471 1.841 2.033 O.D. 0.029 0.127 0.209 0.392 0.731 1.471 1.841 2.033

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.