Mouse E-Cadherin PicoKine® Quick ELISA Kit

Mouse E-Cadherin 1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse Cdh1 in cell culture supernatants, serum and plasma (heparin) . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

E-Cadherin, also called Cadherin 1 (CDH1), Uvomorulin or calcium-dependent ashesion protein, epithelial. E-cadherin is a Ca (2+) -dependent epithelial cell-cell adhesion molecule. Downregulation of E-cadherin expression often correlates with strong invasive potential and poor prognosis of human carcinomas. The gene spans a region of approximately 100 kb, and its location on chromosome 16q22.1. It contains 16 exons and a 65-kb-long intron 2. E-cadherin gene mutations may contribute to the development of diffusely growing gastric carcinomas. E-cadherin plays a central part in the process of epithelial morphogenesis and acts as a strong invasion suppressor in experimental tumor cell systems.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 242 247 246 242 244 2.27 0.9% Sample 2 1644 1884 1788 1601 1729 113.04 6.5% Sample 3 5631 5520 5145 4973 5317 268.19 5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 242 247 246 242 244 2.27 0.9% Sample 1 242 247 246 242 244 2.27 0.9% Sample 2 1644 1884 1788 1601 1729 113.04 6.5% Sample 2 1644 1884 1788 1601 1729 113.04 6.5% Sample 3 5631 5520 5145 4973 5317 268.19 5% Sample 3 5631 5520 5145 4973 5317 268.19 5%

Synonyms

Cadherin-1; ARC-1; Epithelial cadherin; E-cadherin; Uvomorulin; CD324; E-Cad/CTF1; E-Cad/CTF2; E-Cad/CTF3; Cdh1

Gene Name

CDH1

Gene ID

12550

UniProt

P09803

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: D157-V709

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 242 1644 5631 217 1771 5410 Standard deviation 14.52 118.36 360.38 14.53 146.99 394.93 CV (%) 6% 7.2% 6.4% 6.7% 8.3% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 242 1644 5631 217 1771 5410 Standard deviation 14.52 118.36 360.38 14.53 146.99 394.93 CV (%) 6% 7.2% 6.4% 6.7% 8.3% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 242 1644 5631 217 1771 5410 Mean (pg/ml) 242 1644 5631 217 1771 5410 Standard deviation 14.52 118.36 360.38 14.53 146.99 394.93 Standard deviation 14.52 118.36 360.38 14.53 146.99 394.93 CV (%) 6% 7.2% 6.4% 6.7% 8.3% 7.3% CV (%) 6% 7.2% 6.4% 6.7% 8.3% 7.3%

Sensitivity

<15 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

98256 MW

Specificity

Natural and recombinant mouse E-Cadherin

Anticoagulant

Heparin

Gene Name Synonym

Cadherin-1

Protein Name

Cadherin-1

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.030 0.084 0.130 0.215 0.421 0.767 1.326 1.980 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.030 0.084 0.130 0.215 0.421 0.767 1.326 1.980 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.030 0.084 0.130 0.215 0.421 0.767 1.326 1.980 O.D. 0.030 0.084 0.130 0.215 0.421 0.767 1.326 1.980

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.