Human CD44 PicoKine® Quick ELISA Kit

CD44 is an integral cell membrane glycoprotein with a postulated role in matrix adhesion lymphocyte activation and lymph node homing. It is contains 19 exons spanning 50 kb of genomic DNA. In humans, the CD44 antigen is encoded by the CD44 gene on Chromosome 11. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs) . Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 236 217 177 288 230 14.96 6.5% Sample 2 1021 739 1139 725 906 66.5 7.3% Sample 3 3364 4572 3629 2766 3583 138.65 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 236 217 177 288 230 14.96 6.5% Sample 1 236 217 177 288 230 14.96 6.5% Sample 2 1021 739 1139 725 906 66.5 7.3% Sample 2 1021 739 1139 725 906 66.5 7.3% Sample 3 3364 4572 3629 2766 3583 138.65 3.9% Sample 3 3364 4572 3629 2766 3583 138.65 3.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 236 1021 3364 187 656 3242 Standard deviation 16 72.18 226.4 9.26 33.98 226.29 CV (%) 6.8% 7.1% 6.7% 5% 5.2% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 236 1021 3364 187 656 3242 Standard deviation 16 72.18 226.4 9.26 33.98 226.29 CV (%) 6.8% 7.1% 6.7% 5% 5.2% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 236 1021 3364 187 656 3242 Mean (pg/ml) 236 1021 3364 187 656 3242 Standard deviation 16 72.18 226.4 9.26 33.98 226.29 Standard deviation 16 72.18 226.4 9.26 33.98 226.29 CV (%) 6.8% 7.1% 6.7% 5% 5.2% 7% CV (%) 6.8% 7.1% 6.7% 5% 5.2% 7%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.051 0.101 0.134 0.243 0.439 0.829 1.482 2.072 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.051 0.101 0.134 0.243 0.439 0.829 1.482 2.072 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.051 0.101 0.134 0.243 0.439 0.829 1.482 2.072 O.D. 0.051 0.101 0.134 0.243 0.439 0.829 1.482 2.072

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.