Human CD44 PicoKine® Quick ELISA Kit

Human CD44 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human CD44 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CD44 is an integral cell membrane glycoprotein with a postulated role in matrix adhesion lymphocyte activation and lymph node homing. It is contains 19 exons spanning 50 kb of genomic DNA. In humans, the CD44 antigen is encoded by the CD44 gene on Chromosome 11. The protein encoded by this gene is a cell-surface glycoprotein involved in cell-cell interactions, cell adhesion and migration. It is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs) . Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full length nature of some of these variants has not been determined. Alternative splicing is the basis for the structural and functional diversity of this protein, and may be related to tumor metastasis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 236 217 177 288 230 14.96 6.5% Sample 2 1021 739 1139 725 906 66.5 7.3% Sample 3 3364 4572 3629 2766 3583 138.65 3.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 236 217 177 288 230 14.96 6.5% Sample 1 236 217 177 288 230 14.96 6.5% Sample 2 1021 739 1139 725 906 66.5 7.3% Sample 2 1021 739 1139 725 906 66.5 7.3% Sample 3 3364 4572 3629 2766 3583 138.65 3.9% Sample 3 3364 4572 3629 2766 3583 138.65 3.9%

Gene Name

CD44

Gene ID

960

UniProt

P16070

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Gln21-Pro220

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

50 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 236 1021 3364 187 656 3242 Standard deviation 16 72.18 226.4 9.26 33.98 226.29 CV (%) 6.8% 7.1% 6.7% 5% 5.2% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 236 1021 3364 187 656 3242 Standard deviation 16 72.18 226.4 9.26 33.98 226.29 CV (%) 6.8% 7.1% 6.7% 5% 5.2% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 236 1021 3364 187 656 3242 Mean (pg/ml) 236 1021 3364 187 656 3242 Standard deviation 16 72.18 226.4 9.26 33.98 226.29 Standard deviation 16 72.18 226.4 9.26 33.98 226.29 CV (%) 6.8% 7.1% 6.7% 5% 5.2% 7% CV (%) 6.8% 7.1% 6.7% 5% 5.2% 7%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

81538 MW

Specificity

Natural and recombinant human CD44

Anticoagulant

Heparin or EDTA

Gene Name Synonym

CD44 antigen

Protein Name

CD44 antigen

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.051 0.101 0.134 0.243 0.439 0.829 1.482 2.072 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.051 0.101 0.134 0.243 0.439 0.829 1.482 2.072 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.051 0.101 0.134 0.243 0.439 0.829 1.482 2.072 O.D. 0.051 0.101 0.134 0.243 0.439 0.829 1.482 2.072

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.