Rat IL-10 PicoKine® Quick ELISA Kit

Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease. The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 58 57 49 54 3.49 6.4% Sample 2 213 219 232 225 222 7.04 3.1% Sample 3 839 901 925 826 872 41.39 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 58 57 49 54 3.49 6.4% Sample 1 55 58 57 49 54 3.49 6.4% Sample 2 213 219 232 225 222 7.04 3.1% Sample 2 213 219 232 225 222 7.04 3.1% Sample 3 839 901 925 826 872 41.39 4.7% Sample 3 839 901 925 826 872 41.39 4.7%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 213 839 51 222 910 Standard deviation 4.34 9.58 36.07 4.74 12.21 39.13 CV (%) 7.9% 4.5% 4.3% 9.3% 5.5% 4.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 213 839 51 222 910 Standard deviation 4.34 9.58 36.07 4.74 12.21 39.13 CV (%) 7.9% 4.5% 4.3% 9.3% 5.5% 4.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 55 213 839 51 222 910 Mean (pg/ml) 55 213 839 51 222 910 Standard deviation 4.34 9.58 36.07 4.74 12.21 39.13 Standard deviation 4.34 9.58 36.07 4.74 12.21 39.13 CV (%) 7.9% 4.5% 4.3% 9.3% 5.5% 4.3% CV (%) 7.9% 4.5% 4.3% 9.3% 5.5% 4.3%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.059 0.168 0.259 0.466 0.893 1.550 1.843 2.039 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.059 0.168 0.259 0.466 0.893 1.550 1.843 2.039 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.059 0.168 0.259 0.466 0.893 1.550 1.843 2.039 O.D. 0.059 0.168 0.259 0.466 0.893 1.550 1.843 2.039

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.