Rat IL-10 PicoKine® Quick ELISA Kit

Rat IL-10/Interleukin-10 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Rat Il10 in cell culture supernatants, serum and plasma (EDTA) . Sensitivity: 4pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease. The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 58 57 49 54 3.49 6.4% Sample 2 213 219 232 225 222 7.04 3.1% Sample 3 839 901 925 826 872 41.39 4.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 55 58 57 49 54 3.49 6.4% Sample 1 55 58 57 49 54 3.49 6.4% Sample 2 213 219 232 225 222 7.04 3.1% Sample 2 213 219 232 225 222 7.04 3.1% Sample 3 839 901 925 826 872 41.39 4.7% Sample 3 839 901 925 826 872 41.39 4.7%

Synonyms

Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF; Il10; Il-10

Gene Name

IL10

Gene ID

25325

UniProt

P29456

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S19-N178

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (EDTA) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 213 839 51 222 910 Standard deviation 4.34 9.58 36.07 4.74 12.21 39.13 CV (%) 7.9% 4.5% 4.3% 9.3% 5.5% 4.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 55 213 839 51 222 910 Standard deviation 4.34 9.58 36.07 4.74 12.21 39.13 CV (%) 7.9% 4.5% 4.3% 9.3% 5.5% 4.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 55 213 839 51 222 910 Mean (pg/ml) 55 213 839 51 222 910 Standard deviation 4.34 9.58 36.07 4.74 12.21 39.13 Standard deviation 4.34 9.58 36.07 4.74 12.21 39.13 CV (%) 7.9% 4.5% 4.3% 9.3% 5.5% 4.3% CV (%) 7.9% 4.5% 4.3% 9.3% 5.5% 4.3%

Sensitivity

<4 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

20426 MW

Specificity

Natural and recombinant rat IL-10

Anticoagulant

EDTA

Gene Name Synonym

Interleukin-10

Protein Name

Interleukin-10

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.059 0.168 0.259 0.466 0.893 1.550 1.843 2.039 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.059 0.168 0.259 0.466 0.893 1.550 1.843 2.039 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.059 0.168 0.259 0.466 0.893 1.550 1.843 2.039 O.D. 0.059 0.168 0.259 0.466 0.893 1.550 1.843 2.039

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.