Mouse CD40L PicoKine® Quick ELISA Kit

CD40 ligand (CD40L) is a type II membrane protein of 261 amino acids on activated T cells that induces B cell proliferation and immunoglobulin secretion. It has homology with tumour necrosis factor-alpha and -beta, and has important functions in B-cell activation and differentiation. Human CD40L with 5 exons, is mapped to the proximal region of the mouse X chromosome on Xq26.3-27.1, and can be detected on T cells but is absent from B cells and monocytes. Since CD40L is expressed on platelets and released from them on activation, its predictive value as a marker for clinical outcome and the therapeutic effect of inhibition of glycoprotein IIb/IIIa receptor in patients with acute coronary syndromes was investigated. The soluble CD40L may be involved in the process of restenosis and that it exerts its effect by triggering a complex group of inflammatory reactions on endothelial and mononuclear cells.CD40L plays a central role in the pathophysiology of acute coronary syndromes, and has a role in the pathogenesis of coronary artery lesions.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 202 213 180 201 199 11.93 5.9% Sample 2 698 648 727 616 672 43.04 6.4% Sample 3 1026 1040 977 1103 1036 44.95 4.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 202 213 180 201 199 11.93 5.9% Sample 1 202 213 180 201 199 11.93 5.9% Sample 2 698 648 727 616 672 43.04 6.4% Sample 2 698 648 727 616 672 43.04 6.4% Sample 3 1026 1040 977 1103 1036 44.95 4.3% Sample 3 1026 1040 977 1103 1036 44.95 4.3%

CD40 ligand; CD40-L; T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD154; CD40 ligand, membrane form; CD40 ligand, soluble form; Cd40lg; Cd40l, Tnfsf5

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 202 698 1026 185 716 1058 Standard deviation 13.93 39.08 79 14.06 52.26 85.69 CV (%) 6.9% 5.6% 7.7% 7.6% 7.3% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 202 698 1026 185 716 1058 Standard deviation 13.93 39.08 79 14.06 52.26 85.69 CV (%) 6.9% 5.6% 7.7% 7.6% 7.3% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 202 698 1026 185 716 1058 Mean (pg/ml) 202 698 1026 185 716 1058 Standard deviation 13.93 39.08 79 14.06 52.26 85.69 Standard deviation 13.93 39.08 79 14.06 52.26 85.69 CV (%) 6.9% 5.6% 7.7% 7.6% 7.3% 8.1% CV (%) 6.9% 5.6% 7.7% 7.6% 7.3% 8.1%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.055 0.092 0.166 0.229 0.356 0.588 1.016 1.822 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.055 0.092 0.166 0.229 0.356 0.588 1.016 1.822 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.055 0.092 0.166 0.229 0.356 0.588 1.016 1.822 O.D. 0.055 0.092 0.166 0.229 0.356 0.588 1.016 1.822

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.