Mouse CSF1R PicoKine® Quick ELISA Kit

Mouse CSF1R/M-CSFR PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse Csf1r in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

CSF1R (Colony stimulating factor 1 receptor), also known as M-CSFR and CD115, is a cell-surface protein encoded, in humans, by the CSF1R gene. The gene is located on long arm of chromosome 5 (5q32) on the Crick (minus) strand. The encoded protein is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. The encoded protein is a single pass type I membrane protein and acts as the receptor for colony stimulating factor 1, a cytokine which controls the production, differentiation, and function of macrophages. Both CSF1R, and its ligand colony stimulating factor 1 play an important role in the development of the mammary gland and may be involved in the process of mammary gland carcinogenesis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 106 110 121 107 111 5.95 5.3% Sample 2 398 456 393 395 410 26.32 6.4% Sample 3 1799 1871 1812 1936 1854 54.31 2.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 106 110 121 107 111 5.95 5.3% Sample 1 106 110 121 107 111 5.95 5.3% Sample 2 398 456 393 395 410 26.32 6.4% Sample 2 398 456 393 395 410 26.32 6.4% Sample 3 1799 1871 1812 1936 1854 54.31 2.9% Sample 3 1799 1871 1812 1936 1854 54.31 2.9%

Synonyms

Macrophage colony-stimulating factor 1 receptor; CSF-1 receptor; CSF-1-R; CSF-1R; M-CSF-R;2.7.10.1; Proto-oncogene c-Fms; CD115; Csf1r; Csfmr, Fms

Gene Name

CSF1R

Gene ID

12978

UniProt

P09581

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A20-S511

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

62.5 pg/mL - 4,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 106 398 1799 110 427 1748 Standard deviation 8.16 24.27 131.32 10.23 32.87 137.74 CV (%) 7.7% 6.1% 7.3% 9.3% 7.7% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 106 398 1799 110 427 1748 Standard deviation 8.16 24.27 131.32 10.23 32.87 137.74 CV (%) 7.7% 6.1% 7.3% 9.3% 7.7% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 106 398 1799 110 427 1748 Mean (pg/ml) 106 398 1799 110 427 1748 Standard deviation 8.16 24.27 131.32 10.23 32.87 137.74 Standard deviation 8.16 24.27 131.32 10.23 32.87 137.74 CV (%) 7.7% 6.1% 7.3% 9.3% 7.7% 7.8% CV (%) 7.7% 6.1% 7.3% 9.3% 7.7% 7.8%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

109179 MW

Specificity

Natural and recombinant mouse CSF1R

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Macrophage colony-stimulating factor 1 receptor

Protein Name

Macrophage colony-stimulating factor 1 receptor

Sample Data

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.041 0.116 0.162 0.279 0.494 0.835 1.317 1.992 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.041 0.116 0.162 0.279 0.494 0.835 1.317 1.992 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.041 0.116 0.162 0.279 0.494 0.835 1.317 1.992 O.D. 0.041 0.116 0.162 0.279 0.494 0.835 1.317 1.992

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.