Mouse Cathepsin B PicoKine® Quick ELISA Kit

Mouse Cathepsin B PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse Ctsb in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant mouse Cathepsin B with the molecular mass of 37KDa.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 242 214 229 235 230 10.31 4.4% Sample 2 1828 1771 1721 1761 1770 38.23 2.1% Sample 3 4749 4980 5227 4633 4897 227.69 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 242 214 229 235 230 10.31 4.4% Sample 1 242 214 229 235 230 10.31 4.4% Sample 2 1828 1771 1721 1761 1770 38.23 2.1% Sample 2 1828 1771 1721 1761 1770 38.23 2.1% Sample 3 4749 4980 5227 4633 4897 227.69 4.6% Sample 3 4749 4980 5227 4633 4897 227.69 4.6%

Synonyms

Cathepsin B;3.4.22.1; Cathepsin B1; Cathepsin B light chain; Cathepsin B heavy chain; Ctsb

Gene Name

CTSB

Gene ID

13030

UniProt

P10605

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: H18-F339

Capture Antibody

Monoclonal antibody from rat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 242 1828 4749 229 1916 4534 Standard deviation 14.76 100.54 370.42 13.96 128.37 439.79 CV (%) 6.1% 5.5% 7.8% 6.1% 6.7% 9.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 242 1828 4749 229 1916 4534 Standard deviation 14.76 100.54 370.42 13.96 128.37 439.79 CV (%) 6.1% 5.5% 7.8% 6.1% 6.7% 9.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 242 1828 4749 229 1916 4534 Mean (pg/ml) 242 1828 4749 229 1916 4534 Standard deviation 14.76 100.54 370.42 13.96 128.37 439.79 Standard deviation 14.76 100.54 370.42 13.96 128.37 439.79 CV (%) 6.1% 5.5% 7.8% 6.1% 6.7% 9.7% CV (%) 6.1% 5.5% 7.8% 6.1% 6.7% 9.7%

Sensitivity

<5 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

37280 MW

Specificity

Natural and recombinant mouse Cathepsin B

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Cathepsin B

Protein Name

Cathepsin B

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.068 0.118 0.221 0.458 0.949 1.601 2.087 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.068 0.118 0.221 0.458 0.949 1.601 2.087 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.068 0.118 0.221 0.458 0.949 1.601 2.087 O.D. 0.006 0.068 0.118 0.221 0.458 0.949 1.601 2.087

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.