Rat DKK-1 PicoKine® Quick ELISA Kit

Rat DKK-1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Rat Dkk1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 91 93 93 92 92 0.82 0.8% Sample 2 361 310 365 327 340 23.09 6.7% Sample 3 1121 1338 1162 1204 1206 81.53 6.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 91 93 93 92 92 0.82 0.8% Sample 1 91 93 93 92 92 0.82 0.8% Sample 2 361 310 365 327 340 23.09 6.7% Sample 2 361 310 365 327 340 23.09 6.7% Sample 3 1121 1338 1162 1204 1206 81.53 6.7% Sample 3 1121 1338 1162 1204 1206 81.53 6.7%

Synonyms

Dickkopf homolog 1 (Xenopus laevis) (Predicted) ; Dickkopf-like protein 1 ; Protein Dkk1 ; Dkk1 ; DKK1 , Dkk1_predicted , LOC100912050 ; rCG_48380

Gene Name

Dkk1

Gene ID

293897

UniProt

D3Z9J1

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: S23-H270

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

46.9 pg/mL - 3,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 91 361 1121 84 359 1024 Standard deviation 6.46 28.51 88.4%9 7.39 38.4%8 86.01 CV (%) 7.1% 7.9% 7.6% 8.8% 9.8% 8.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 91 361 1121 84 359 1024 Standard deviation 6.46 28.51 88.4%9 7.39 38.4%8 86.01 CV (%) 7.1% 7.9% 7.6% 8.8% 9.8% 8.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 91 361 1121 84 359 1024 Mean (pg/ml) 91 361 1121 84 359 1024 Standard deviation 6.46 28.51 88.4%9 7.39 38.4%8 86.01 Standard deviation 6.46 28.51 88.4%9 7.39 38.4%8 86.01 CV (%) 7.1% 7.9% 7.6% 8.8% 9.8% 8.4% CV (%) 7.1% 7.9% 7.6% 8.8% 9.8% 8.4%

Sensitivity

<15 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

29149 MW

Specificity

Natural and recombinant rat DKK1

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Dickkopf WNT signaling pathway inhibitor 1

Protein Name

Dickkopf homolog 1

Sample Data

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.010 0.089 0.136 0.243 0.464 0.862 1.376 2.000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.010 0.089 0.136 0.243 0.464 0.862 1.376 2.000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.010 0.089 0.136 0.243 0.464 0.862 1.376 2.000 O.D. 0.010 0.089 0.136 0.243 0.464 0.862 1.376 2.000

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.