Rat CCL4/MIP1β PicoKine® Quick ELISA Kit

Rat CCL4/MIP-1 Beta PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Rat Ccl4 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in mouse is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. By analysis of somatic cell hybrids and by in situ hybridization, the CCL4 gene was assigned to 17q21-q23. Based on functional analysis of MIP-1beta, one or more additional beta chemokine receptors that transduce MIP-1beta signals must also exist. MIP-1alpha and MIP-1beta have been shown to lack affinity for the erythrocyte surface chemokine receptor/Duffy antigen that binds many alpha as well as beta chemokines (11, 14) .

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 27 31 27 29 2 6.8% Sample 2 144 160 147 149 150 6.04 4% Sample 3 453 458 508 520 484 29.6 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 31 27 31 27 29 2 6.8% Sample 1 31 27 31 27 29 2 6.8% Sample 2 144 160 147 149 150 6.04 4% Sample 2 144 160 147 149 150 6.04 4% Sample 3 453 458 508 520 484 29.6 6.1% Sample 3 453 458 508 520 484 29.6 6.1%

Synonyms

C-C motif chemokine 4; Macrophage inflammatory protein 1-beta; MIP-1-beta; Small-inducible cytokine A4; Ccl4; Mip1b, Scya4

Gene Name

CCL4

Gene ID

116637

UniProt

P50230

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A24-N92

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 144 453 31 148 419 Standard deviation 1.42 9.21 24.31 1.67 12.13 24.3 CV (%) 4.6% 6.4% 5.5% 5.4% 8.2% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 31 144 453 31 148 419 Standard deviation 1.42 9.21 24.31 1.67 12.13 24.3 CV (%) 4.6% 6.4% 5.5% 5.4% 8.2% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 31 144 453 31 148 419 Mean (pg/ml) 31 144 453 31 148 419 Standard deviation 1.42 9.21 24.31 1.67 12.13 24.3 Standard deviation 1.42 9.21 24.31 1.67 12.13 24.3 CV (%) 4.6% 6.4% 5.5% 5.4% 8.2% 5.8% CV (%) 4.6% 6.4% 5.5% 5.4% 8.2% 5.8%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

10234 MW

Specificity

Natural and recombinant rat CCL4

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

C-C motif chemokine 4

Protein Name

C-C motif chemokine 4

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.018 0.096 0.162 0.336 0.636 1.267 1.691 2.040 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.018 0.096 0.162 0.336 0.636 1.267 1.691 2.040 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.018 0.096 0.162 0.336 0.636 1.267 1.691 2.040 O.D. 0.018 0.096 0.162 0.336 0.636 1.267 1.691 2.040

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.