Mouse CD80/B7-1 PicoKine® Quick ELISA Kit

The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7.1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain. The CD80 and CD86 (601020) genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4. The standard product used in this kit is recombinant B7-1, D37—K245, which is composed of two single chains acids with the dipolymer.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 153 139 133 151 144 8.3 5.7% Sample 2 733 831 739 836 784 48.82 6.2% Sample 3 1704 1609 1450 1522 1571 95.09 6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 153 139 133 151 144 8.3 5.7% Sample 1 153 139 133 151 144 8.3 5.7% Sample 2 733 831 739 836 784 48.82 6.2% Sample 2 733 831 739 836 784 48.82 6.2% Sample 3 1704 1609 1450 1522 1571 95.09 6% Sample 3 1704 1609 1450 1522 1571 95.09 6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 153 733 1704 158 717 1630 Standard deviation 9.94 47.64 132.91 13.27 59.51 133.66 CV (%) 6.5% 6.5% 7.8% 8.4% 8.3% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 153 733 1704 158 717 1630 Standard deviation 9.94 47.64 132.91 13.27 59.51 133.66 CV (%) 6.5% 6.5% 7.8% 8.4% 8.3% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 153 733 1704 158 717 1630 Mean (pg/ml) 153 733 1704 158 717 1630 Standard deviation 9.94 47.64 132.91 13.27 59.51 133.66 Standard deviation 9.94 47.64 132.91 13.27 59.51 133.66 CV (%) 6.5% 6.5% 7.8% 8.4% 8.3% 8.2% CV (%) 6.5% 6.5% 7.8% 8.4% 8.3% 8.2%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.031 0.060 0.103 0.206 0.398 0.849 1.561 2.089 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.031 0.060 0.103 0.206 0.398 0.849 1.561 2.089 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.031 0.060 0.103 0.206 0.398 0.849 1.561 2.089 O.D. 0.031 0.060 0.103 0.206 0.398 0.849 1.561 2.089

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.