Human CA9 PicoKine® Quick ELISA Kit

Carbonic anhydrase 9 (CA9/CAIX) is an enzyme that in humans is encoded by the CA9 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene is mapped to 9p13.3. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 32 32 33 33 2.06 6.2% Sample 2 196 230 215 208 212 12.29 5.7% Sample 3 412 423 456 402 423 20.31 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 37 32 32 33 33 2.06 6.2% Sample 1 37 32 32 33 33 2.06 6.2% Sample 2 196 230 215 208 212 12.29 5.7% Sample 2 196 230 215 208 212 12.29 5.7% Sample 3 412 423 456 402 423 20.31 4.8% Sample 3 412 423 456 402 423 20.31 4.8%

Carbonic anhydrase 9;4.2.1.1; Carbonate dehydratase IX; Carbonic anhydrase IX; CA-IX; CAIX; Membrane antigen MN; P54/58N; Renal cell carcinoma-associated antigen G250; RCC-associated antigen G250; pMW1; CA9; G250, MN

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 196 412 33 176 428 Standard deviation 2.84 10.97 27%3 2.77 11.61 29.96 CV (%) 7.7% 5.6% 6.1% 8.4% 6.6% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37 196 412 33 176 428 Standard deviation 2.84 10.97 27%3 2.77 11.61 29.96 CV (%) 7.7% 5.6% 6.1% 8.4% 6.6% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 37 196 412 33 176 428 Mean (pg/ml) 37 196 412 33 176 428 Standard deviation 2.84 10.97 27%3 2.77 11.61 29.96 Standard deviation 2.84 10.97 27%3 2.77 11.61 29.96 CV (%) 7.7% 5.6% 6.1% 8.4% 6.6% 7% CV (%) 7.7% 5.6% 6.1% 8.4% 6.6% 7%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.038 0.098 0.155 0.270 0.549 1.051 1.778 2.064 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.038 0.098 0.155 0.270 0.549 1.051 1.778 2.064 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.038 0.098 0.155 0.270 0.549 1.051 1.778 2.064 O.D. 0.038 0.098 0.155 0.270 0.549 1.051 1.778 2.064

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.