Rat ICAM-1 PicoKine® Quick ELISA Kit

Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1) . ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses. The standard product used in this kit is produced by recombinant gene expression, which is a dimeric protein linked with disulfide bonds.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 51 47 49 56 50 3.34 6.6% Sample 2 231 227 275 247 245 18.86 7.6% Sample 3 787 761 862 797 801 37.18 4.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 51 47 49 56 50 3.34 6.6% Sample 1 51 47 49 56 50 3.34 6.6% Sample 2 231 227 275 247 245 18.86 7.6% Sample 2 231 227 275 247 245 18.86 7.6% Sample 3 787 761 862 797 801 37.18 4.6% Sample 3 787 761 862 797 801 37.18 4.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 51 231 787 51 242 855 Standard deviation 3.67 14.55 42.49 4.23 16.21 51.3 CV (%) 7.2% 6.7% 5.4% 8.3% 6.7% 6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 51 231 787 51 242 855 Standard deviation 3.67 14.55 42.49 4.23 16.21 51.3 CV (%) 7.2% 6.7% 5.4% 8.3% 6.7% 6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 51 231 787 51 242 855 Mean (pg/ml) 51 231 787 51 242 855 Standard deviation 3.67 14.55 42.49 4.23 16.21 51.3 Standard deviation 3.67 14.55 42.49 4.23 16.21 51.3 CV (%) 7.2% 6.7% 5.4% 8.3% 6.7% 6% CV (%) 7.2% 6.7% 5.4% 8.3% 6.7% 6%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.055 0.128 0.214 0.338 0.633 1.195 1.918 1.995 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.055 0.128 0.214 0.338 0.633 1.195 1.918 1.995 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.055 0.128 0.214 0.338 0.633 1.195 1.918 1.995 O.D. 0.055 0.128 0.214 0.338 0.633 1.195 1.918 1.995

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.