Human RAGE PicoKine® Quick ELISA Kit

Human Rage PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Human AGER in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

RAGE, the Receptor for Advanced Glycation Endproducts, is a 35kD transmembrane receptor of the immunoglobulin super family. It is also known as “AGER”. AGER gene is mapped to chromosome 6p21.3 by mapping by contiguous cosmids and YAC clones and by fluorescence in situ hybridization. The expression of RAGE is particularly increased in neurons close to deposits of amyloid beta peptide and to neurofibrillary tangles. RAGE has been linked to several chronic diseases, which are thought to result from vascular damage. The pathogenesis is hypothesized to include ligand binding upon which RAGE signals activation of the nuclear factor kappa B (NF-kappaB) .

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 154 153 141 152 150 5.24 3.4% Sample 2 623 570 627 596 604 22.96 3.8% Sample 3 3076 3035 3093 3144 3087 39.08 1.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 154 153 141 152 150 5.24 3.4% Sample 1 154 153 141 152 150 5.24 3.4% Sample 2 623 570 627 596 604 22.96 3.8% Sample 2 623 570 627 596 604 22.96 3.8% Sample 3 3076 3035 3093 3144 3087 39.08 1.2% Sample 3 3076 3035 3093 3144 3087 39.08 1.2%

Synonyms

CDNA FLJ56412, highly similar to Advanced glycosylation end product-specific receptor

Gene Name

AGER

Gene ID

177

UniProt

B4DNX3

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q24-A344

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

78 pg/mL - 5,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 154 623 3076 159 595 3344 Standard deviation 10.16 27.41 221.47 10.65 32.72 250.8 CV (%) 6.6% 4.4% 7.2% 6.7% 5.5% 7.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 154 623 3076 159 595 3344 Standard deviation 10.16 27.41 221.47 10.65 32.72 250.8 CV (%) 6.6% 4.4% 7.2% 6.7% 5.5% 7.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 154 623 3076 159 595 3344 Mean (pg/ml) 154 623 3076 159 595 3344 Standard deviation 10.16 27.41 221.47 10.65 32.72 250.8 Standard deviation 10.16 27.41 221.47 10.65 32.72 250.8 CV (%) 6.6% 4.4% 7.2% 6.7% 5.5% 7.5% CV (%) 6.6% 4.4% 7.2% 6.7% 5.5% 7.5%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Calculated Molecular Weight

45980 MW

Specificity

Natural and recombinant human Rage

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Advanced glycosylation end product-specific receptor

Protein Name

Advanced glycosylation end product-specific receptor

Sample Data

Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.037 0.116 0.191 0.330 0.611 1.112 1.761 2.023 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.037 0.116 0.191 0.330 0.611 1.112 1.761 2.023 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 312 625 1250 2500 5000 O.D. 0.037 0.116 0.191 0.330 0.611 1.112 1.761 2.023 O.D. 0.037 0.116 0.191 0.330 0.611 1.112 1.761 2.023

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.