Human ACE PicoKine® Quick ELISA Kit

Angiotensin-converting enzyme (ACE) is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. The predicted protein is identical, from residue 37 to its C terminus, to the second half or C-terminal domain of the endothelial ACE sequence. The protein sequence inferred consists of a 732-residue preprotein including a 31-residue signal peptide. The mature polypeptide has a molecular weight of 80,073. Although ACE has been studied primarily in the context of its role in blood pressure regulation, this widely distributed enzyme has many other physiological functions. The ACE gene encodes two isozymes. The somatic isozyme is expressed in many tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells, whereas the testicular or germinal angiotensin-converting enzyme is expressed only in sperm. The standard product used in this kit is recombinant human ACE, consisting of 30-1261 amino acids with the molecular mass of 120KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1278 1310 1292 1425 1326 58.13 4.3% Sample 2 6839 6994 7039 6669 6885 145.23 2.1% Sample 3 24157 24884 22207 21600 23212 1350.66 5.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1278 1310 1292 1425 1326 58.13 4.3% Sample 1 1278 1310 1292 1425 1326 58.13 4.3% Sample 2 6839 6994 7039 6669 6885 145.23 2.1% Sample 2 6839 6994 7039 6669 6885 145.23 2.1% Sample 3 24157 24884 22207 21600 23212 1350.66 5.8% Sample 3 24157 24884 22207 21600 23212 1350.66 5.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1278 6839 24157 1358 6350 25528 Standard deviation 62.62 465.05 1087.06 86.57 482.6 1225.34 CV (%) 4.9% 6.8% 4.5% 6.5% 7.6% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1278 6839 24157 1358 6350 25528 Standard deviation 62.62 465.05 1087.06 86.57 482.6 1225.34 CV (%) 4.9% 6.8% 4.5% 6.5% 7.6% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1278 6839 24157 1358 6350 25528 Mean (pg/ml) 1278 6839 24157 1358 6350 25528 Standard deviation 62.62 465.05 1087.06 86.57 482.6 1225.34 Standard deviation 62.62 465.05 1087.06 86.57 482.6 1225.34 CV (%) 4.9% 6.8% 4.5% 6.5% 7.6% 4.8% CV (%) 4.9% 6.8% 4.5% 6.5% 7.6% 4.8%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.037 0.109 0.201 0.357 0.614 1.113 1.750 2.023 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.037 0.109 0.201 0.357 0.614 1.113 1.750 2.023 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.037 0.109 0.201 0.357 0.614 1.113 1.750 2.023 O.D. 0.037 0.109 0.201 0.357 0.614 1.113 1.750 2.023

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.