Mouse IGF-2 PicoKine® Quick ELISA Kit

Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 83 84 91 92 87 4.03 4.6% Sample 2 719 780 698 710 726 31.63 4.3% Sample 3 980 989 1144 1081 1048 67.83 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 83 84 91 92 87 4.03 4.6% Sample 1 83 84 91 92 87 4.03 4.6% Sample 2 719 780 698 710 726 31.63 4.3% Sample 2 719 780 698 710 726 31.63 4.3% Sample 3 980 989 1144 1081 1048 67.83 6.4% Sample 3 980 989 1144 1081 1048 67.83 6.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 83 719 980 84 692 946 Standard deviation 5.89 56.08 61.74 6.55 63.66 73.78 CV (%) 7.1% 7.8% 9.2% 7.8% 9.2% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 83 719 980 84 692 946 Standard deviation 5.89 56.08 61.74 6.55 63.66 73.78 CV (%) 7.1% 7.8% 9.2% 7.8% 9.2% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 83 719 980 84 692 946 Mean (pg/ml) 83 719 980 84 692 946 Standard deviation 5.89 56.08 61.74 6.55 63.66 73.78 Standard deviation 5.89 56.08 61.74 6.55 63.66 73.78 CV (%) 7.1% 7.8% 9.2% 7.8% 9.2% 7.8% CV (%) 7.1% 7.8% 9.2% 7.8% 9.2% 7.8%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.065 0.133 0.190 0.316 0.589 1.117 1.885 2.032 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.065 0.133 0.190 0.316 0.589 1.117 1.885 2.032 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.065 0.133 0.190 0.316 0.589 1.117 1.885 2.032 O.D. 0.065 0.133 0.190 0.316 0.589 1.117 1.885 2.032

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.