Human IFN Gamma/IFNG/Interferon Gamma PicoKine® Quick ELISA Kit

Human IFN Gamma/IFNG/Interferon Gamma PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate human IFNG in cell culture supernatants and serum. Sensitivity: 2 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Interferon-gamma (IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer (NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 38 37 36 36 1.47 4% Sample 2 150 152 164 166 158 7.07 4.4% Sample 3 469 446 458 468 460 9.28 2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 34 38 37 36 36 1.47 4% Sample 1 34 38 37 36 36 1.47 4% Sample 2 150 152 164 166 158 7.07 4.4% Sample 2 150 152 164 166 158 7.07 4.4% Sample 3 469 446 458 468 460 9.28 2% Sample 3 469 446 458 468 460 9.28 2%

Gene Name

IFNG

Gene ID

3458

UniProt

P01579

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q24-Q166

Capture Antibody

MonEK0373oclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants and serum.

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 150 469 33 139 437 Standard deviation 1.73 8.7 29.54 1.91 9.73 31.9 CV (%) 7.3% 5.8% 7% 5.8% 7% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 34 150 469 33 139 437 Standard deviation 1.73 8.7 29.54 1.91 9.73 31.9 CV (%) 7.3% 5.8% 7% 5.8% 7% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 34 150 469 33 139 437 Mean (pg/ml) 34 150 469 33 139 437 Standard deviation 1.73 8.7 29.54 1.91 9.73 31.9 Standard deviation 1.73 8.7 29.54 1.91 9.73 31.9 CV (%) 7.3% 5.8% 7% 5.8% 7% 7.3% CV (%) 7.3% 5.8% 7% 5.8% 7% 7.3%

Sensitivity

<2 pg/mL

Components

Description|Quantity|Volume Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 HRP-Linked Antibody|1|6ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.023 0.098 0.167 0.314 0.595 0.748 1.846 2.068 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.023 0.098 0.167 0.314 0.595 0.748 1.846 2.068 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.023 0.098 0.167 0.314 0.595 0.748 1.846 2.068 O.D. 0.023 0.098 0.167 0.314 0.595 0.748 1.846 2.068

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.