Human CCL18/PARC PicoKine® Quick ELISA Kit

Chemokine (C-C motif) ligand 18 (CCL18) is a small cytokine belonging to the CC chemokine family that was previously called PARC (pulmonary and activation-regulated chemokine) . CCL18 is approximately 60% identical in amino acid sequence to CCL3. By analysis of a previously mapped CCL18 from 17q11.2, it is determined that the PARC gene is located within 1 of the 2 clusters of CC chemokine genes in this region. It is expressed at high levels in lung and at lower levels in certain lymphoid tissues, such as the lymph nodes, and is chemotactic for activated T cells and nonactivated lymphocytes. Beside, CCL18 recruits Th2 cells and basophils and may play a predominant role in allergic asthma.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 17 18 18 17 0.43 2.5% Sample 2 49 56 49 48 50 3.2 6.4% Sample 3 269 295 278 296 284 11.45 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 18 17 18 18 17 0.43 2.5% Sample 1 18 17 18 18 17 0.43 2.5% Sample 2 49 56 49 48 50 3.2 6.4% Sample 2 49 56 49 48 50 3.2 6.4% Sample 3 269 295 278 296 284 11.45 4% Sample 3 269 295 278 296 284 11.45 4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 49 269 18 52 279 Standard deviation 0.99 3.52 20.17 1.29 4.62 22.59 CV (%) 5.5% 7.2% 7.5% 7.2% 8.9% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 18 49 269 18 52 279 Standard deviation 0.99 3.52 20.17 1.29 4.62 22.59 CV (%) 5.5% 7.2% 7.5% 7.2% 8.9% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 18 49 269 18 52 279 Mean (pg/ml) 18 49 269 18 52 279 Standard deviation 0.99 3.52 20.17 1.29 4.62 22.59 Standard deviation 0.99 3.52 20.17 1.29 4.62 22.59 CV (%) 5.5% 7.2% 7.5% 7.2% 8.9% 8.1% CV (%) 5.5% 7.2% 7.5% 7.2% 8.9% 8.1%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.033 0.100 0.147 0.261 0.446 0.854 1.582 2.058 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.033 0.100 0.147 0.261 0.446 0.854 1.582 2.058 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 Concentration (pg/ml) 0 7.8 15.6 31.3 62.5 125 250 500 O.D. 0.033 0.100 0.147 0.261 0.446 0.854 1.582 2.058 O.D. 0.033 0.100 0.147 0.261 0.446 0.854 1.582 2.058

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.