Mouse Angiopoietin-1 PicoKine® Quick ELISA Kit

Mouse Angiopoietin-1 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate mouse Angpt1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 311 293 312 282 299 12.61 4.2% Sample 2 1285 1071 1253 1292 1225 90.26 7.3% Sample 3 4930 5240 5287 4371 4957 365.07 7.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 311 293 312 282 299 12.61 4.2% Sample 1 311 293 312 282 299 12.61 4.2% Sample 2 1285 1071 1253 1292 1225 90.26 7.3% Sample 2 1285 1071 1253 1292 1225 90.26 7.3% Sample 3 4930 5240 5287 4371 4957 365.07 7.3% Sample 3 4930 5240 5287 4371 4957 365.07 7.3%

Gene Name

ANGPT1

Gene ID

11600

UniProt

O08538

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: H16-F497

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) .

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 311 1285 4930 287 1289 5041 Standard deviation 18.97 60.39 295.8 18.65 70.89 317.58 CV (%) 6.1% 4.7% 6% 6.5% 5.5% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 311 1285 4930 287 1289 5041 Standard deviation 18.97 60.39 295.8 18.65 70.89 317.58 CV (%) 6.1% 4.7% 6% 6.5% 5.5% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 311 1285 4930 287 1289 5041 Mean (pg/ml) 311 1285 4930 287 1289 5041 Standard deviation 18.97 60.39 295.8 18.65 70.89 317.58 Standard deviation 18.97 60.39 295.8 18.65 70.89 317.58 CV (%) 6.1% 4.7% 6% 6.5% 5.5% 6.3% CV (%) 6.1% 4.7% 6% 6.5% 5.5% 6.3%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 156 313 625 1250 2500 5000 10000 O.D. 0.084 0.147 0.222 0.338 0.568 1.017 1.673 2.044 Concentration (pg/ml) 0 156 313 625 1250 2500 5000 10000 O.D. 0.084 0.147 0.222 0.338 0.568 1.017 1.673 2.044 Concentration (pg/ml) 0 156 313 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 313 625 1250 2500 5000 10000 O.D. 0.084 0.147 0.222 0.338 0.568 1.017 1.673 2.044 O.D. 0.084 0.147 0.222 0.338 0.568 1.017 1.673 2.044

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.