Human CXCL5/ENA-78 PicoKine® Quick ELISA Kit

Human CXCL5/ENA-78 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate human CXCL5 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78) . It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 66 77 65 68 69 4.74 6.8% Sample 2 270 257 287 288 275 12.85 4.6% Sample 3 964 912 900 813 897 54.26 6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 66 77 65 68 69 4.74 6.8% Sample 1 66 77 65 68 69 4.74 6.8% Sample 2 270 257 287 288 275 12.85 4.6% Sample 2 270 257 287 288 275 12.85 4.6% Sample 3 964 912 900 813 897 54.26 6% Sample 3 964 912 900 813 897 54.26 6%

Gene Name

CXCL5

Gene ID

6374

UniProt

P42830

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A37-N114

Capture Antibody

Monoclonal antibody from mouse

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tubex2

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate) .

Detection Range

31.2 pg/mL - 2,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 66 270 964 61 293 970 Standard deviation 2.77 18.09 74.22 3.53 22.26 82.45 CV (%) 4.2% 6.7% 7.7% 5.8% 7.6% 8.5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 66 270 964 61 293 970 Standard deviation 2.77 18.09 74.22 3.53 22.26 82.45 CV (%) 4.2% 6.7% 7.7% 5.8% 7.6% 8.5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 66 270 964 61 293 970 Mean (pg/ml) 66 270 964 61 293 970 Standard deviation 2.77 18.09 74.22 3.53 22.26 82.45 Standard deviation 2.77 18.09 74.22 3.53 22.26 82.45 CV (%) 4.2% 6.7% 7.7% 5.8% 7.6% 8.5% CV (%) 4.2% 6.7% 7.7% 5.8% 7.6% 8.5%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Anticoagulant

Heparin, EDTA or citrate

Sample Data

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.022 0.161 0.306 0.614 1.143 1.877 2.081 2.097 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.022 0.161 0.306 0.614 1.143 1.877 2.081 2.097 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1,000 2,000 O.D. 0.022 0.161 0.306 0.614 1.143 1.877 2.081 2.097 O.D. 0.022 0.161 0.306 0.614 1.143 1.877 2.081 2.097

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.