Human MMP-2 PicoKine® Quick ELISA Kit

Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 952 1166 1130 693 985 50.15 5.1% Sample 2 1335 2149 1773 1456 1668 39.37 2.4% Sample 3 18623 16083 10251 14876 14868 793.96 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 952 1166 1130 693 985 50.15 5.1% Sample 1 952 1166 1130 693 985 50.15 5.1% Sample 2 1335 2149 1773 1456 1668 39.37 2.4% Sample 2 1335 2149 1773 1456 1668 39.37 2.4% Sample 3 18623 16083 10251 14876 14868 793.96 5.3% Sample 3 18623 16083 10251 14876 14868 793.96 5.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 952 1335 18263 716 1356 10353 Standard deviation 53.03 97.05 1313.11 18.83 58.99 792 CV (%) 5.6% 7.3% 7.2% 2.6% 4.4% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 952 1335 18263 716 1356 10353 Standard deviation 53.03 97.05 1313.11 18.83 58.99 792 CV (%) 5.6% 7.3% 7.2% 2.6% 4.4% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 952 1335 18263 716 1356 10353 Mean (pg/ml) 952 1335 18263 716 1356 10353 Standard deviation 53.03 97.05 1313.11 18.83 58.99 792 Standard deviation 53.03 97.05 1313.11 18.83 58.99 792 CV (%) 5.6% 7.3% 7.2% 2.6% 4.4% 7.7% CV (%) 5.6% 7.3% 7.2% 2.6% 4.4% 7.7%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (pg/ml) 0 625 1,250 2,500 5,000 10,000 20,000 40,000 O.D. 0.015 0.190 0.335 0.627 1.138 1.738 1.905 2.079 Concentration (pg/ml) 0 625 1,250 2,500 5,000 10,000 20,000 40,000 O.D. 0.015 0.190 0.335 0.627 1.138 1.738 1.905 2.079 Concentration (pg/ml) 0 625 1,250 2,500 5,000 10,000 20,000 40,000 Concentration (pg/ml) 0 625 1,250 2,500 5,000 10,000 20,000 40,000 O.D. 0.015 0.190 0.335 0.627 1.138 1.738 1.905 2.079 O.D. 0.015 0.190 0.335 0.627 1.138 1.738 1.905 2.079

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.