Human MEP1A ELISA Kit PicoKine®

Meprin A subunit alpha also known as endopeptidase-2 or PABA peptide hydrolase is the alpha subunit of the meprin A enzyme that in humans is encoded by the MEP1A gene. Meprin A subunit alpha, encoded by the **MEP1A** gene, is a component of the meprin A metalloproteinase complex, which is involved in extracellular matrix remodeling and proteolysis. Meprin A is a zinc-dependent endopeptidase found predominantly in the kidneys, intestines, and other tissues. It plays a crucial role in various physiological processes, including inflammation, tissue repair, and the processing of bioactive peptides. The alpha subunit, specifically, is a part of the hetero-oligomeric enzyme complex, which combines with the beta subunit to form an active enzyme. Abnormalities in meprin A function have been associated with several pathological conditions, including kidney diseases and cancer.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2306 2413 2894 2515 2532 97.23 3.8% Sample 2 10121 8303 10346 10403 9793 553.32 5.7% Sample 3 43453 33164 27466 33589 34418 2725.91 7.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 2306 2413 2894 2515 2532 97.23 3.8% Sample 1 2306 2413 2894 2515 2532 97.23 3.8% Sample 2 10121 8303 10346 10403 9793 553.32 5.7% Sample 2 10121 8303 10346 10403 9793 553.32 5.7% Sample 3 43453 33164 27466 33589 34418 2725.91 7.9% Sample 3 43453 33164 27466 33589 34418 2725.91 7.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2306 10121 43453 2328 7457 37974 Standard deviation 163.03 401.8 2342.12 153.18 501.11 2529.07 CV (%) 7.1% 4% 5.4% 6.6% 6.7% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2306 10121 43453 2328 7457 37974 Standard deviation 163.03 401.8 2342.12 153.18 501.11 2529.07 CV (%) 7.1% 4% 5.4% 6.6% 6.7% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2306 10121 43453 2328 7457 37974 Mean (pg/ml) 2306 10121 43453 2328 7457 37974 Standard deviation 163.03 401.8 2342.12 153.18 501.11 2529.07 Standard deviation 163.03 401.8 2342.12 153.18 501.11 2529.07 CV (%) 7.1% 4% 5.4% 6.6% 6.7% 6.7% CV (%) 7.1% 4% 5.4% 6.6% 6.7% 6.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|6

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.026 0.114 0.189 0.32 0.605 1.111 1.812 2.522 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.026 0.114 0.189 0.32 0.605 1.111 1.812 2.522 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.026 0.114 0.189 0.32 0.605 1.111 1.812 2.522 O.D. 0.026 0.114 0.189 0.32 0.605 1.111 1.812 2.522

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.