Human C-Peptide ELISA Kit PicoKine®

C-Peptide, or connecting peptide, is a short peptide chain that is produced during the synthesis of insulin. When proinsulin is cleaved to form insulin, C-Peptide is released as a byproduct. It serves as a useful biomarker for assessing insulin production in the body because its levels reflect the amount of insulin secreted by the pancreas. Unlike insulin, C-Peptide has a longer half-life in the bloodstream, making it easier to measure. Elevated levels of C-Peptide can indicate insulinoma or insulin resistance, while low levels may suggest type 1 diabetes or pancreatic dysfunction. Understanding C-Peptide levels can help in diagnosing and managing various metabolic disorders.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 69 72 106 71 80 5.23 6.6% Sample 2 318 500 455 447 430 15.01 3.5% Sample 3 1720 1706 1260 1331 1504 117.63 7.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 69 72 106 71 80 5.23 6.6% Sample 1 69 72 106 71 80 5.23 6.6% Sample 2 318 500 455 447 430 15.01 3.5% Sample 2 318 500 455 447 430 15.01 3.5% Sample 3 1720 1706 1260 1331 1504 117.63 7.8% Sample 3 1720 1706 1260 1331 1504 117.63 7.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 69 318 1720 92 351 1533 Standard deviation 1.6 17.84 50.05 4.66 13.3 66.84 CV (%) 2.3% 5.6% 2.9% 5.1% 3.8% 4.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 69 318 1720 92 351 1533 Standard deviation 1.6 17.84 50.05 4.66 13.3 66.84 CV (%) 2.3% 5.6% 2.9% 5.1% 3.8% 4.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 69 318 1720 92 351 1533 Mean (pg/ml) 69 318 1720 92 351 1533 Standard deviation 1.6 17.84 50.05 4.66 13.3 66.84 Standard deviation 1.6 17.84 50.05 4.66 13.3 66.84 CV (%) 2.3% 5.6% 2.9% 5.1% 3.8% 4.4% CV (%) 2.3% 5.6% 2.9% 5.1% 3.8% 4.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|5

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.045 0.122 0.21 0.368 0.654 1.089 1.651 2.237 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.045 0.122 0.21 0.368 0.654 1.089 1.651 2.237 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.045 0.122 0.21 0.368 0.654 1.089 1.651 2.237 O.D. 0.045 0.122 0.21 0.368 0.654 1.089 1.651 2.237

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.