Human/Canine/Porcine Insulin ELISA Kit PicoKine®

Insulin is a peptide hormone produced by beta cells of the pancreatic islets encoded in humans by the insulin (INS) gene. This gene encodes insulin, a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified, including insulin-dependent diabetes mellitus, permanent neonatal diabetes diabetes mellitus, maturity-onset diabetes of the young type 10 and hyperproinsulinemia. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region.

Lots Lot 1 (pmol/L) Lot 2 (pmol/L) Lot 3 (pmol/L) Lot 4 (pmol/L) Mean (pmol/L) Standard Deviation CV (%) Sample 1 28 18 24 19 22 1.69 7.6% Sample 2 93 93 112 85 96 6.96 7.3% Sample 3 474 411 299 444 407 21.08 5.2% Lots Lot 1 (pmol/L) Lot 2 (pmol/L) Lot 3 (pmol/L) Lot 4 (pmol/L) Mean (pmol/L) Standard Deviation CV (%) Lots Lot 1 (pmol/L) Lot 2 (pmol/L) Lot 3 (pmol/L) Lot 4 (pmol/L) Mean (pmol/L) Standard Deviation CV (%) Sample 1 28 18 24 19 22 1.69 7.6% Sample 1 28 18 24 19 22 1.69 7.6% Sample 2 93 93 112 85 96 6.96 7.3% Sample 2 93 93 112 85 96 6.96 7.3% Sample 3 474 411 299 444 407 21.08 5.2% Sample 3 474 411 299 444 407 21.08 5.2%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pmol/L) 28 93 474 25 118 274 Standard deviation 1.53 2.72 10.57 1.14 5.17 21.81 CV (%) 5.5% 2.9% 2.2% 4.5% 4.4% 8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pmol/L) 28 93 474 25 118 274 Standard deviation 1.53 2.72 10.57 1.14 5.17 21.81 CV (%) 5.5% 2.9% 2.2% 4.5% 4.4% 8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pmol/L) 28 93 474 25 118 274 Mean (pmol/L) 28 93 474 25 118 274 Standard deviation 1.53 2.72 10.57 1.14 5.17 21.81 Standard deviation 1.53 2.72 10.57 1.14 5.17 21.81 CV (%) 5.5% 2.9% 2.2% 4.5% 4.4% 8% CV (%) 5.5% 2.9% 2.2% 4.5% 4.4% 8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pmol/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.004 0.086 0.184 0.294 0.56 0.989 1.621 2.158 Concentration (pmol/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.004 0.086 0.184 0.294 0.56 0.989 1.621 2.158 Concentration (pmol/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pmol/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.004 0.086 0.184 0.294 0.56 0.989 1.621 2.158 O.D. 0.004 0.086 0.184 0.294 0.56 0.989 1.621 2.158

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.