Mouse IFN-Beta ELISA Kit PicoKine®

Interferon beta is a protein that in humans is encoded by the IFNB1 gene. This gene encodes a cytokine that belongs to the interferon family of signaling proteins, which are released as part of the innate immune response to pathogens. The protein encoded by this gene belongs to the type I class of interferons, which are important for defense against viral infections. In addition, type I interferons are involved in cell differentiation and anti-tumor defenses. Following secretion in response to a pathogen, type I interferons bind a homologous receptor complex and induce transcription of genes such as those encoding inflammatory cytokines and chemokines. Overactivation of type I interferon secretion is linked to autoimmune diseases. Mice deficient for this gene display several phenotypes including defects in B cell maturation and increased susceptibility to viral infection.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 72 95 107 70 86 3.76 4.4% Sample 2 354 365 401 288 352 14.89 4.2% Sample 3 1168 1375 1157 1831 1383 74.53 5.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 72 95 107 70 86 3.76 4.4% Sample 1 72 95 107 70 86 3.76 4.4% Sample 2 354 365 401 288 352 14.89 4.2% Sample 2 354 365 401 288 352 14.89 4.2% Sample 3 1168 1375 1157 1831 1383 74.53 5.4% Sample 3 1168 1375 1157 1831 1383 74.53 5.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 72 354 1168 78 459 1393 Standard deviation 5.66 22.62 67.98 3.08 36.72 91.8 CV (%) 7.9% 6.4% 5.8% 4% 8% 6.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 72 354 1168 78 459 1393 Standard deviation 5.66 22.62 67.98 3.08 36.72 91.8 CV (%) 7.9% 6.4% 5.8% 4% 8% 6.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 72 354 1168 78 459 1393 Mean (pg/ml) 72 354 1168 78 459 1393 Standard deviation 5.66 22.62 67.98 3.08 36.72 91.8 Standard deviation 5.66 22.62 67.98 3.08 36.72 91.8 CV (%) 7.9% 6.4% 5.8% 4% 8% 6.6% CV (%) 7.9% 6.4% 5.8% 4% 8% 6.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.1 0.17 0.297 0.537 0.97 1.806 2.443 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.1 0.17 0.297 0.537 0.97 1.806 2.443 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.008 0.1 0.17 0.297 0.537 0.97 1.806 2.443 O.D. 0.008 0.1 0.17 0.297 0.537 0.97 1.806 2.443

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.