Human ECM1 ELISA Kit PicoKine®

Extracellular matrix protein 1 is a protein that in humans is encoded by the ECM1 gene. This gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 251 301 306 301 290 17.65 6.1% Sample 2 1041 946 960 1121 1017 40.99 4% Sample 3 3816 2746 4780 2745 3522 215.88 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 251 301 306 301 290 17.65 6.1% Sample 1 251 301 306 301 290 17.65 6.1% Sample 2 1041 946 960 1121 1017 40.99 4% Sample 2 1041 946 960 1121 1017 40.99 4% Sample 3 3816 2746 4780 2745 3522 215.88 6.1% Sample 3 3816 2746 4780 2745 3522 215.88 6.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 251 1041 3816 180 1013 4617 Standard deviation 18.9 32.79 140.81 13.75 27.76 211.92 CV (%) 7.5% 3.2% 3.7% 7.6% 2.7% 4.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 251 1041 3816 180 1013 4617 Standard deviation 18.9 32.79 140.81 13.75 27.76 211.92 CV (%) 7.5% 3.2% 3.7% 7.6% 2.7% 4.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 251 1041 3816 180 1013 4617 Mean (pg/ml) 251 1041 3816 180 1013 4617 Standard deviation 18.9 32.79 140.81 13.75 27.76 211.92 Standard deviation 18.9 32.79 140.81 13.75 27.76 211.92 CV (%) 7.5% 3.2% 3.7% 7.6% 2.7% 4.6% CV (%) 7.5% 3.2% 3.7% 7.6% 2.7% 4.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.03 0.148 0.269 0.429 0.747 1.178 1.665 2.031 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.03 0.148 0.269 0.429 0.747 1.178 1.665 2.031 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.03 0.148 0.269 0.429 0.747 1.178 1.665 2.031 O.D. 0.03 0.148 0.269 0.429 0.747 1.178 1.665 2.031

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.