Human OSMRb ELISA Kit PicoKine®

Osteoclasts are multinucleated cells that resorb bone and are essential for bone homeostasis. This gene encodes an osteoclast-associated receptor (OSCAR), which is a member of the leukocyte receptor complex protein family that plays critical roles in the regulation of both innate and adaptive immune responses. The encoded protein may play a role in oxidative stress-mediated atherogenesis as well as monocyte adhesion. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 307 165 291 270 258 6.95 2.7% Sample 2 496 458 396 543 473 22.86 4.8% Sample 3 3021 2785 3127 3535 3117 237.2 7.6% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 307 165 291 270 258 6.95 2.7% Sample 1 307 165 291 270 258 6.95 2.7% Sample 2 496 458 396 543 473 22.86 4.8% Sample 2 496 458 396 543 473 22.86 4.8% Sample 3 3021 2785 3127 3535 3117 237.2 7.6% Sample 3 3021 2785 3127 3535 3117 237.2 7.6%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 307 496 3021 265 461 3015 Standard deviation 22.07 32.98 80.36 18.47 14.11 173.97 CV (%) 7.2% 6.7% 2.7% 7% 3.1% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 307 496 3021 265 461 3015 Standard deviation 22.07 32.98 80.36 18.47 14.11 173.97 CV (%) 7.2% 6.7% 2.7% 7% 3.1% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 307 496 3021 265 461 3015 Mean (pg/ml) 307 496 3021 265 461 3015 Standard deviation 22.07 32.98 80.36 18.47 14.11 173.97 Standard deviation 22.07 32.98 80.36 18.47 14.11 173.97 CV (%) 7.2% 6.7% 2.7% 7% 3.1% 5.8% CV (%) 7.2% 6.7% 2.7% 7% 3.1% 5.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.138 0.259 0.419 0.743 1.162 1.676 2.03 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.138 0.259 0.419 0.743 1.162 1.676 2.03 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.025 0.138 0.259 0.419 0.743 1.162 1.676 2.03 O.D. 0.025 0.138 0.259 0.419 0.743 1.162 1.676 2.03

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.