Human MD2 ELISA Kit PicoKine®

Lymphocyte antigen 96, also known as Myeloid Differentiation factor 2 (MD-2), is a protein that in humans is encoded by the LY96 gene. This gene encodes a protein which associates with toll-like receptor 4 on the cell surface and confers responsiveness to lipopolysaccyaride (LPS), thus providing a link between the receptor and LPS signaling. Studies of the mouse ortholog suggest that this gene may be involved in endotoxin neutralization. Alternative splicing results in multiple transcript variants encoding different isoforms.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 168 178 143 111 150 6.87 4.6% Sample 2 354 344 240 308 312 16.67 5.4% Sample 3 2287 1955 1800 2074 2029 155.42 7.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 168 178 143 111 150 6.87 4.6% Sample 1 168 178 143 111 150 6.87 4.6% Sample 2 354 344 240 308 312 16.67 5.4% Sample 2 354 344 240 308 312 16.67 5.4% Sample 3 2287 1955 1800 2074 2029 155.42 7.7% Sample 3 2287 1955 1800 2074 2029 155.42 7.7%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 168 354 2287 130 244 1770 Standard deviation 6.69 16.43 166.49 3.17 15.05 60 CV (%) 4% 4.6% 7.3% 2.4% 6.2% 3.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 168 354 2287 130 244 1770 Standard deviation 6.69 16.43 166.49 3.17 15.05 60 CV (%) 4% 4.6% 7.3% 2.4% 6.2% 3.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 168 354 2287 130 244 1770 Mean (pg/ml) 168 354 2287 130 244 1770 Standard deviation 6.69 16.43 166.49 3.17 15.05 60 Standard deviation 6.69 16.43 166.49 3.17 15.05 60 CV (%) 4% 4.6% 7.3% 2.4% 6.2% 3.4% CV (%) 4% 4.6% 7.3% 2.4% 6.2% 3.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.012 0.104 0.189 0.399 0.723 1.132 1.815 2.168 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.012 0.104 0.189 0.399 0.723 1.132 1.815 2.168 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.012 0.104 0.189 0.399 0.723 1.132 1.815 2.168 O.D. 0.012 0.104 0.189 0.399 0.723 1.132 1.815 2.168

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.