Human MEPE ELISA Kit PicoKine®

Matrix extracellular phosphoglycoprotein (Osteoblast/osteocyte factor 45) is a protein that in humans is encoded by the MEPE gene. This gene encodes a secreted calcium-binding phosphoprotein that belongs to the small integrin-binding ligand, N-linked glycoprotein (SIBLING) family of proteins. Members of this family are components of the extracellular matrix of bone and dentin and regulate bone mineralization. Deficiency of a similar protein in mouse results in increased bone mass. Mice lacking this gene are resistant to aging-related trabecular bone loss. Alternative splicing results in multiple transcript variants.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 224 226 307 234 248 18.01 7.3% Sample 2 462 335 340 457 399 19.57 4.9% Sample 3 4230 4343 3648 4613 4209 247.88 5.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 224 226 307 234 248 18.01 7.3% Sample 1 224 226 307 234 248 18.01 7.3% Sample 2 462 335 340 457 399 19.57 4.9% Sample 2 462 335 340 457 399 19.57 4.9% Sample 3 4230 4343 3648 4613 4209 247.88 5.9% Sample 3 4230 4343 3648 4613 4209 247.88 5.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 224 462 4230 205 609 3785 Standard deviation 15.39 18.94 157.78 15.95 19.37 123.39 CV (%) 6.9% 4.1% 3.7% 7.8% 3.2% 3.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 224 462 4230 205 609 3785 Standard deviation 15.39 18.94 157.78 15.95 19.37 123.39 CV (%) 6.9% 4.1% 3.7% 7.8% 3.2% 3.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 224 462 4230 205 609 3785 Mean (pg/ml) 224 462 4230 205 609 3785 Standard deviation 15.39 18.94 157.78 15.95 19.37 123.39 Standard deviation 15.39 18.94 157.78 15.95 19.37 123.39 CV (%) 6.9% 4.1% 3.7% 7.8% 3.2% 3.3% CV (%) 6.9% 4.1% 3.7% 7.8% 3.2% 3.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.173 0.288 0.454 0.765 1.204 1.674 2.046 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.173 0.288 0.454 0.765 1.204 1.674 2.046 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.173 0.288 0.454 0.765 1.204 1.674 2.046 O.D. 0.049 0.173 0.288 0.454 0.765 1.204 1.674 2.046

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.