Human MEGF9 ELISA Kit PicoKine®

MEGF9 (Multiple EGF?like domains protein 9; also EGF?like protein 5) is a 63 kDa (predicted) novel transmembrane glycoprotein that shares some homology to beta?chains of laminin. It is expressed by hepatocytes, cerebellar Purkinje cells, Schwann cells, keratinocytes and intestinal epithelium. MEGF9 is suggested to participate in cell motility, and its absence correlates with tumor cell migration. Mature human MEGF9 is 572 amino acids (aa) in length. It is a single span type I transmembrane protein that contains a 484 aa extracellular region (aa 31?514) plus a 68 aa C?terminal cytoplasmic domain. The extracellular region possesses a lengthy Pro?rich region (aa 55?200), followed by five EGF?like domains (aa 204?451) . MEGF9 may run at approximately 160 kDa in SDS?PAGE, suggesting either heavy glycosylation or dimerization. Over aa 36?514, human MEGF9 shares 76% aa sequence identity with mouse MEGF9.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 223 215 175 306 230 16.96 7.4% Sample 2 315 457 345 413 383 26.12 6.8% Sample 3 3772 4291 2918 4826 3952 199.17 5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 223 215 175 306 230 16.96 7.4% Sample 1 223 215 175 306 230 16.96 7.4% Sample 2 315 457 345 413 383 26.12 6.8% Sample 2 315 457 345 413 383 26.12 6.8% Sample 3 3772 4291 2918 4826 3952 199.17 5% Sample 3 3772 4291 2918 4826 3952 199.17 5%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 223 315 3772 266 463 4194 Standard deviation 12.93 19.47 117.31 5.61 36.3 249.54 CV (%) 5.8% 6.2% 3.1% 2.1% 7.8% 6.0% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 223 315 3772 266 463 4194 Standard deviation 12.93 19.47 117.31 5.61 36.3 249.54 CV (%) 5.8% 6.2% 3.1% 2.1% 7.8% 6.0% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 223 315 3772 266 463 4194 Mean (pg/ml) 223 315 3772 266 463 4194 Standard deviation 12.93 19.47 117.31 5.61 36.3 249.54 Standard deviation 12.93 19.47 117.31 5.61 36.3 249.54 CV (%) 5.8% 6.2% 3.1% 2.1% 7.8% 6.0% CV (%) 5.8% 6.2% 3.1% 2.1% 7.8% 6.0%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.152 0.267 0.434 0.752 1.177 1.679 2.039 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.152 0.267 0.434 0.752 1.177 1.679 2.039 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.035 0.152 0.267 0.434 0.752 1.177 1.679 2.039 O.D. 0.035 0.152 0.267 0.434 0.752 1.177 1.679 2.039

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.