Human NQO1 ELISA Kit PicoKine®

NAD (P) H dehydrogenase [quinone] 1 is an enzyme that in humans is encoded by the NQO1 gene. This gene is a member of the NAD (P) H dehydrogenase (quinone) family and encodes a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. This protein's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Mutations in this gene have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. Altered expression of this protein has been seen in many tumors and is also associated with Alzheimer's disease (AD) . Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 17 20 23 17 19 1.18 6.1% Sample 2 59 51 59 54 56 3.23 5.8% Sample 3 426 399 478 329 408 20.64 5.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 17 20 23 17 19 1.18 6.1% Sample 1 17 20 23 17 19 1.18 6.1% Sample 2 59 51 59 54 56 3.23 5.8% Sample 2 59 51 59 54 56 3.23 5.8% Sample 3 426 399 478 329 408 20.64 5.1% Sample 3 426 399 478 329 408 20.64 5.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 17 59 426 25 52 251 Standard deviation 0.85 3.15 32.04 0.77 3.66 11.47 CV (%) 5% 5.3% 7.5% 3.1% 7% 4.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 17 59 426 25 52 251 Standard deviation 0.85 3.15 32.04 0.77 3.66 11.47 CV (%) 5% 5.3% 7.5% 3.1% 7% 4.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 17 59 426 25 52 251 Mean (pg/ml) 17 59 426 25 52 251 Standard deviation 0.85 3.15 32.04 0.77 3.66 11.47 Standard deviation 0.85 3.15 32.04 0.77 3.66 11.47 CV (%) 5% 5.3% 7.5% 3.1% 7% 4.6% CV (%) 5% 5.3% 7.5% 3.1% 7% 4.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.062 0.132 0.197 0.31 0.543 0.917 1.383 1.977 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.062 0.132 0.197 0.31 0.543 0.917 1.383 1.977 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.062 0.132 0.197 0.31 0.543 0.917 1.383 1.977 O.D. 0.062 0.132 0.197 0.31 0.543 0.917 1.383 1.977

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.