Human NPTXR ELISA Kit PicoKine®

Neuronal pentraxin receptor is a protein that in humans is encoded by the NPTXR gene. This gene encodes a protein similar to the rat neuronal pentraxin receptor. The rat pentraxin receptor is an integral membrane protein that is thought to mediate neuronal uptake of the snake venom toxin, taipoxin, and its transport into the synapses. Studies in rat indicate that translation of this mRNA initiates at a non-AUG (CUG) codon. This may also be true for mouse and human, based on strong sequence conservation amongst these species.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 85 95 69 72 80 1.66 2.1% Sample 2 217 230 192 226 216 15.66 7.2% Sample 3 1469 1243 1953 1586 1563 35.47 2.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 85 95 69 72 80 1.66 2.1% Sample 1 85 95 69 72 80 1.66 2.1% Sample 2 217 230 192 226 216 15.66 7.2% Sample 2 217 230 192 226 216 15.66 7.2% Sample 3 1469 1243 1953 1586 1563 35.47 2.3% Sample 3 1469 1243 1953 1586 1563 35.47 2.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 85 217 1469 116 186 1697 Standard deviation 6.52 12.78 114.14 5.06 7.66 40.73 CV (%) 7.7% 5.9% 7.8% 4.4% 4.1% 2.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 85 217 1469 116 186 1697 Standard deviation 6.52 12.78 114.14 5.06 7.66 40.73 CV (%) 7.7% 5.9% 7.8% 4.4% 4.1% 2.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 85 217 1469 116 186 1697 Mean (pg/ml) 85 217 1469 116 186 1697 Standard deviation 6.52 12.78 114.14 5.06 7.66 40.73 Standard deviation 6.52 12.78 114.14 5.06 7.66 40.73 CV (%) 7.7% 5.9% 7.8% 4.4% 4.1% 2.4% CV (%) 7.7% 5.9% 7.8% 4.4% 4.1% 2.4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.002 0.062 0.117 0.238 0.467 0.8 1.443 2.038 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.002 0.062 0.117 0.238 0.467 0.8 1.443 2.038 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.002 0.062 0.117 0.238 0.467 0.8 1.443 2.038 O.D. 0.002 0.062 0.117 0.238 0.467 0.8 1.443 2.038

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.