Human NOTCH3 ELISA Kit PicoKine®

Neurogenic locus notch homolog protein 3 (Notch 3) is a protein that in humans is encoded by the NOTCH3 gene. This gene encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) .

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 70 60 85 52 67 2.73 4.1% Sample 2 171 126 181 177 164 8.7 5.3% Sample 3 1100 1319 1144 911 1119 83.22 7.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 70 60 85 52 67 2.73 4.1% Sample 1 70 60 85 52 67 2.73 4.1% Sample 2 171 126 181 177 164 8.7 5.3% Sample 2 171 126 181 177 164 8.7 5.3% Sample 3 1100 1319 1144 911 1119 83.22 7.4% Sample 3 1100 1319 1144 911 1119 83.22 7.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 70 171 1100 65 148 845 Standard deviation 3.89 13.68 84.07 1.37 6.72 47.49 CV (%) 5.6% 8% 7.4% 2.1% 4.5% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 70 171 1100 65 148 845 Standard deviation 3.89 13.68 84.07 1.37 6.72 47.49 CV (%) 5.6% 8% 7.4% 2.1% 4.5% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 70 171 1100 65 148 845 Mean (pg/ml) 70 171 1100 65 148 845 Standard deviation 3.89 13.68 84.07 1.37 6.72 47.49 Standard deviation 3.89 13.68 84.07 1.37 6.72 47.49 CV (%) 5.6% 8% 7.4% 2.1% 4.5% 5.6% CV (%) 5.6% 8% 7.4% 2.1% 4.5% 5.6%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.045 0.089 0.127 0.211 0.393 0.678 1.323 2.204 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.045 0.089 0.127 0.211 0.393 0.678 1.323 2.204 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.045 0.089 0.127 0.211 0.393 0.678 1.323 2.204 O.D. 0.045 0.089 0.127 0.211 0.393 0.678 1.323 2.204

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.