Human MFAP5 ELISA Kit PicoKine®

Microfibrillar-associated protein 5 is a protein that in humans is encoded by the MFAP5 gene. This gene encodes a 25-kD microfibril-associated glycoprotein which is a component of microfibrils of the extracellular matrix. The encoded protein promotes attachment of cells to microfibrils via alpha-V-beta-3 integrin. Deficiency of this gene in mice results in neutropenia. Alternate splicing results in multiple transcript variants encoding different isoforms.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 306 178 273 175 233 14.17 6.1% Sample 2 393 415 409 410 407 24.57 6% Sample 3 3203 4322 4523 4416 4116 179.05 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 306 178 273 175 233 14.17 6.1% Sample 1 306 178 273 175 233 14.17 6.1% Sample 2 393 415 409 410 407 24.57 6% Sample 2 393 415 409 410 407 24.57 6% Sample 3 3203 4322 4523 4416 4116 179.05 4.4% Sample 3 3203 4322 4523 4416 4116 179.05 4.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 306 393 3203 267 394 3107 Standard deviation 7.47 13.32 94.49 14.85 14.38 122.73 CV (%) 2.4% 3.4% 3% 5.6% 3.7% 4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 306 393 3203 267 394 3107 Standard deviation 7.47 13.32 94.49 14.85 14.38 122.73 CV (%) 2.4% 3.4% 3% 5.6% 3.7% 4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 306 393 3203 267 394 3107 Mean (pg/ml) 306 393 3203 267 394 3107 Standard deviation 7.47 13.32 94.49 14.85 14.38 122.73 Standard deviation 7.47 13.32 94.49 14.85 14.38 122.73 CV (%) 2.4% 3.4% 3% 5.6% 3.7% 4% CV (%) 2.4% 3.4% 3% 5.6% 3.7% 4%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.124 0.247 0.403 0.722 1.157 1.636 2.003 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.124 0.247 0.403 0.722 1.157 1.636 2.003 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.006 0.124 0.247 0.403 0.722 1.157 1.636 2.003 O.D. 0.006 0.124 0.247 0.403 0.722 1.157 1.636 2.003

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.