Human Olfactomedin-2/Noelin-2 ELISA Kit PicoKine®

Human Olfactomedin-2/Noelin-2 ELISA Kit PicoKine® (96 Tests) . Quantitate Human Olfactomedin-2/Noelin-2 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 25 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Olfactomedin 2, also known as noelin 2, is a protein that in humans is encoded by the OLFM2 gene. OLFM2 is an olfactomedin domain-containing protein that is involved in early eye development and function. It also plays a role in TGFB-mediated differentiation of smooth muscle cells (SMCs) from mesenchymal stem cells.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 49 62 49 64 56 1.25 2.2% Sample 2 139 107 110 187 136 4.91 3.6% Sample 3 970 911 1371 1205 1114 71.87 6.5% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 49 62 49 64 56 1.25 2.2% Sample 1 49 62 49 64 56 1.25 2.2% Sample 2 139 107 110 187 136 4.91 3.6% Sample 2 139 107 110 187 136 4.91 3.6% Sample 3 970 911 1371 1205 1114 71.87 6.5% Sample 3 970 911 1371 1205 1114 71.87 6.5%

Gene Name

OLFM2

Gene ID

93145

UniProt

O95897

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q21-P454

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

46.9 pg/mL - 3,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 49 139 970 59 123 838 Standard deviation 1.86 6.27 46.66 2.94 7.49 31.26 CV (%) 3.8% 4.5% 4.8% 5% 6.1% 3.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 49 139 970 59 123 838 Standard deviation 1.86 6.27 46.66 2.94 7.49 31.26 CV (%) 3.8% 4.5% 4.8% 5% 6.1% 3.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 49 139 970 59 123 838 Mean (pg/ml) 49 139 970 59 123 838 Standard deviation 1.86 6.27 46.66 2.94 7.49 31.26 Standard deviation 1.86 6.27 46.66 2.94 7.49 31.26 CV (%) 3.8% 4.5% 4.8% 5% 6.1% 3.7% CV (%) 3.8% 4.5% 4.8% 5% 6.1% 3.7%

Sensitivity

<25 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human OLFM2

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.056 0.076 0.122 0.201 0.387 0.784 1.608 2.584 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.056 0.076 0.122 0.201 0.387 0.784 1.608 2.584 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.056 0.076 0.122 0.201 0.387 0.784 1.608 2.584 O.D. 0.056 0.076 0.122 0.201 0.387 0.784 1.608 2.584

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.