Rat CD25/IL2RA ELISA Kit PicoKine®

CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 114 154 167 139 144 4.69 3.3% Sample 2 306 243 257 203 252 19.55 7.8% Sample 3 2233 2818 2456 2488 2499 151.67 6.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 114 154 167 139 144 4.69 3.3% Sample 1 114 154 167 139 144 4.69 3.3% Sample 2 306 243 257 203 252 19.55 7.8% Sample 2 306 243 257 203 252 19.55 7.8% Sample 3 2233 2818 2456 2488 2499 151.67 6.1% Sample 3 2233 2818 2456 2488 2499 151.67 6.1%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 114 306 2233 155 316 2793 Standard deviation 6.68 15.67 64.09 10.37 22.03 161.99 CV (%) 5.9% 5.1% 2.9% 6.7% 7% 5.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 114 306 2233 155 316 2793 Standard deviation 6.68 15.67 64.09 10.37 22.03 161.99 CV (%) 5.9% 5.1% 2.9% 6.7% 7% 5.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 114 306 2233 155 316 2793 Mean (pg/ml) 114 306 2233 155 316 2793 Standard deviation 6.68 15.67 64.09 10.37 22.03 161.99 Standard deviation 6.68 15.67 64.09 10.37 22.03 161.99 CV (%) 5.9% 5.1% 2.9% 6.7% 7% 5.8% CV (%) 5.9% 5.1% 2.9% 6.7% 7% 5.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.004 0.066 0.118 0.245 0.501 0.811 1.36 1.904 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.004 0.066 0.118 0.245 0.501 0.811 1.36 1.904 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.004 0.066 0.118 0.245 0.501 0.811 1.36 1.904 O.D. 0.004 0.066 0.118 0.245 0.501 0.811 1.36 1.904

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.