Human NUDT5 ELISA Kit PicoKine®

ADP-sugar pyrophosphatase is an enzyme that in humans is encoded by the NUDT5 gene. This gene belongs to the Nudix (nucleoside diphosphate linked moiety X) hydrolase superfamily. The encoded enzyme catalyzes the hydrolysis of modified nucleoside diphosphates, including ADP-ribose (ADPR) and 8-oxoGua-containing 8-oxo-dADP and 8-oxo-dGDP. Protein-bound ADP ribose can be hazardous to the cell because it can modify some amino acid residues, resulting in the inhibition of ATP-activated potassium channels. 8-oxoGua is an oxidized form of guanine that can potentially alter genetic information by pairing with adenine and cytosine in RNA. Presence of 8-oxoGua in RNA results in formation of abnormal proteins due to translational errors.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 111 176 163 134 146 9.31 6.4% Sample 2 340 285 323 278 307 18.94 6.2% Sample 3 2386 2373 2686 2721 2542 174.09 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 111 176 163 134 146 9.31 6.4% Sample 1 111 176 163 134 146 9.31 6.4% Sample 2 340 285 323 278 307 18.94 6.2% Sample 2 340 285 323 278 307 18.94 6.2% Sample 3 2386 2373 2686 2721 2542 174.09 6.9% Sample 3 2386 2373 2686 2721 2542 174.09 6.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 111 340 2386 187 216 2743 Standard deviation 7.51 8.06 132.42 7.16 8.79 74.88 CV (%) 6.8% 2.4% 5.6% 3.8% 4.1% 2.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 111 340 2386 187 216 2743 Standard deviation 7.51 8.06 132.42 7.16 8.79 74.88 CV (%) 6.8% 2.4% 5.6% 3.8% 4.1% 2.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 111 340 2386 187 216 2743 Mean (pg/ml) 111 340 2386 187 216 2743 Standard deviation 7.51 8.06 132.42 7.16 8.79 74.88 Standard deviation 7.51 8.06 132.42 7.16 8.79 74.88 CV (%) 6.8% 2.4% 5.6% 3.8% 4.1% 2.7% CV (%) 6.8% 2.4% 5.6% 3.8% 4.1% 2.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.027 0.121 0.197 0.342 0.649 1.054 1.647 2.039 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.027 0.121 0.197 0.342 0.649 1.054 1.647 2.039 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.027 0.121 0.197 0.342 0.649 1.054 1.647 2.039 O.D. 0.027 0.121 0.197 0.342 0.649 1.054 1.647 2.039

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.