Human OSMR ELISA Kit PicoKine®

Oncostatin-M specific receptor subunit beta also known as the Oncostatin M receptor (OSMR), is one of the receptor proteins for oncostatin M, that in humans is encoded by the OSMR gene. This gene encodes a member of the type I cytokine receptor family. The encoded protein heterodimerizes with interleukin 6 signal transducer to form the type II oncostatin M receptor and with interleukin 31 receptor A to form the interleukin 31 receptor, and thus transduces oncostatin M and interleukin 31 induced signaling events. Mutations in this gene have been associated with familial primary localized cutaneous amyloidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 539 492 621 395 512 35.72 7% Sample 2 665 1230 1094 1027 1004 76.2 7.6% Sample 3 5156 8763 6735 7219 6968 448.76 6.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 539 492 621 395 512 35.72 7% Sample 1 539 492 621 395 512 35.72 7% Sample 2 665 1230 1094 1027 1004 76.2 7.6% Sample 2 665 1230 1094 1027 1004 76.2 7.6% Sample 3 5156 8763 6735 7219 6968 448.76 6.4% Sample 3 5156 8763 6735 7219 6968 448.76 6.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 539 665 5156 442 1131 5693 Standard deviation 40.8 50.34 242.33 12.33 48.18 290.91 CV (%) 7.6% 7.6% 4.7% 2.8% 4.3% 5.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 539 665 5156 442 1131 5693 Standard deviation 40.8 50.34 242.33 12.33 48.18 290.91 CV (%) 7.6% 7.6% 4.7% 2.8% 4.3% 5.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 539 665 5156 442 1131 5693 Mean (pg/ml) 539 665 5156 442 1131 5693 Standard deviation 40.8 50.34 242.33 12.33 48.18 290.91 Standard deviation 40.8 50.34 242.33 12.33 48.18 290.91 CV (%) 7.6% 7.6% 4.7% 2.8% 4.3% 5.1% CV (%) 7.6% 7.6% 4.7% 2.8% 4.3% 5.1%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.028 0.095 0.134 0.21 0.412 0.743 1.365 2.188 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.028 0.095 0.134 0.21 0.412 0.743 1.365 2.188 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.028 0.095 0.134 0.21 0.412 0.743 1.365 2.188 O.D. 0.028 0.095 0.134 0.21 0.412 0.743 1.365 2.188

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.