Human PGLYRP1 ELISA Kit PicoKine®

Peptidoglycan recognition protein 1, PGLYRP1, also known as TAG7, is an antibacterial and pro-inflammatory innate immunity protein that in humans is encoded by the PGLYRP1 gene. Peptidoglycan recognition proteins (PGRPs) are molecules that recognize peptidoglycan, a large component in bacterial cell walls. In insects, PGRPs activate antimicrobial pathways, and in mammals PGRPs function as antibacterial neutrophil proteins. PGRP-L halts bacterial growth by acting as an alanine amidase, an enzyme that hydrolyzes the amide bond of bacterial peptidoglycan. PGRP-I and PGRP-I∫ are also members of the PGRP family that help recognize bacteria by binding to peptidoglycan and Gram-positive bacteria, but they do not have amidase activity. PGRP-S participates in intracellular killing of Gram-positive bacteria by stimulating two antimicrobial defense systems, the prophenoloxidase cascade and the antimicrobial peptides through Toll receptors.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 445 618 349 462 469 9.93 2.1% Sample 2 711 1177 669 1013 893 32.84 3.7% Sample 3 6529 6675 9166 7358 7432 579.7 7.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 445 618 349 462 469 9.93 2.1% Sample 1 445 618 349 462 469 9.93 2.1% Sample 2 711 1177 669 1013 893 32.84 3.7% Sample 2 711 1177 669 1013 893 32.84 3.7% Sample 3 6529 6675 9166 7358 7432 579.7 7.8% Sample 3 6529 6675 9166 7358 7432 579.7 7.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 445 711 6529 564 859 6803 Standard deviation 12.95 34.13 241.57 41.45 26.2 357.16 CV (%) 2.9% 4.8% 3.7% 7.4% 3.1% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 445 711 6529 564 859 6803 Standard deviation 12.95 34.13 241.57 41.45 26.2 357.16 CV (%) 2.9% 4.8% 3.7% 7.4% 3.1% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 445 711 6529 564 859 6803 Mean (pg/ml) 445 711 6529 564 859 6803 Standard deviation 12.95 34.13 241.57 41.45 26.2 357.16 Standard deviation 12.95 34.13 241.57 41.45 26.2 357.16 CV (%) 2.9% 4.8% 3.7% 7.4% 3.1% 5.3% CV (%) 2.9% 4.8% 3.7% 7.4% 3.1% 5.3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.042 0.107 0.16 0.271 0.464 0.911 1.573 2.256 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.042 0.107 0.16 0.271 0.464 0.911 1.573 2.256 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.042 0.107 0.16 0.271 0.464 0.911 1.573 2.256 O.D. 0.042 0.107 0.16 0.271 0.464 0.911 1.573 2.256

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.