Human PEAR1 ELISA Kit PicoKine®

Human PEAR1 ELISA Kit PicoKine® (96 Tests) . Quantitate Human PEAR1 in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 150 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

PEAR1 is a platelet receptor that signals upon the formation of platelet-platelet contacts independent of platelet activation and secondary to platelet aggregation.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 526 341 576 388 458 28.06 6.1% Sample 2 1057 774 955 1025 953 55.93 5.9% Sample 3 7376 9829 5207 8940 7838 581.58 7.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 526 341 576 388 458 28.06 6.1% Sample 1 526 341 576 388 458 28.06 6.1% Sample 2 1057 774 955 1025 953 55.93 5.9% Sample 2 1057 774 955 1025 953 55.93 5.9% Sample 3 7376 9829 5207 8940 7838 581.58 7.4% Sample 3 7376 9829 5207 8940 7838 581.58 7.4%

Gene Name

PEAR1

Gene ID

375033

UniProt

Q5VY43

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: CHO; Immunogen sequence: L21-S754

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 526 1057 7376 503 771 6486 Standard deviation 34.51 37.1 478.7 16.05 60.75 323.65 CV (%) 6.6% 6.5% 6.5% 3.2% 7.9% 5% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 526 1057 7376 503 771 6486 Standard deviation 34.51 37.1 478.7 16.05 60.75 323.65 CV (%) 6.6% 6.5% 6.5% 3.2% 7.9% 5% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 526 1057 7376 503 771 6486 Mean (pg/ml) 526 1057 7376 503 771 6486 Standard deviation 34.51 37.1 478.7 16.05 60.75 323.65 Standard deviation 34.51 37.1 478.7 16.05 60.75 323.65 CV (%) 6.6% 6.5% 6.5% 3.2% 7.9% 5% CV (%) 6.6% 6.5% 6.5% 3.2% 7.9% 5%

Sensitivity

<150 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human PEAR1

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.015 0.09 0.152 0.288 0.55 0.927 1.539 2.114 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.015 0.09 0.152 0.288 0.55 0.927 1.539 2.114 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.015 0.09 0.152 0.288 0.55 0.927 1.539 2.114 O.D. 0.015 0.09 0.152 0.288 0.55 0.927 1.539 2.114

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.