Human PDGFRA ELISA Kit PicoKine®

Platelet-derived growth factor receptor A, also termed CD140a, is a receptor located on the surface of a wide range of cell types. The protein is encoded in the human by the PDGFRA gene. This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies suggest that this gene plays a role in organ development, wound healing, and tumor progression. Mutations in this gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 127 183 95 142 137 4.39 3.2% Sample 2 317 299 328 202 287 9.43 3.3% Sample 3 2663 1607 1919 1566 1939 132.42 6.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 127 183 95 142 137 4.39 3.2% Sample 1 127 183 95 142 137 4.39 3.2% Sample 2 317 299 328 202 287 9.43 3.3% Sample 2 317 299 328 202 287 9.43 3.3% Sample 3 2663 1607 1919 1566 1939 132.42 6.8% Sample 3 2663 1607 1919 1566 1939 132.42 6.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 127 317 2663 109 345 2513 Standard deviation 2.63 16.52 154.72 3.71 23.87 79.66 CV (%) 2.1% 5.2% 5.8% 3.4% 6.9% 3.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 127 317 2663 109 345 2513 Standard deviation 2.63 16.52 154.72 3.71 23.87 79.66 CV (%) 2.1% 5.2% 5.8% 3.4% 6.9% 3.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 127 317 2663 109 345 2513 Mean (pg/ml) 127 317 2663 109 345 2513 Standard deviation 2.63 16.52 154.72 3.71 23.87 79.66 Standard deviation 2.63 16.52 154.72 3.71 23.87 79.66 CV (%) 2.1% 5.2% 5.8% 3.4% 6.9% 3.2% CV (%) 2.1% 5.2% 5.8% 3.4% 6.9% 3.2%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.024 0.089 0.154 0.244 0.471 0.823 1.249 1.939 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.024 0.089 0.154 0.244 0.471 0.823 1.249 1.939 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.8 187.5 375 750 1500 3000 6000 O.D. 0.024 0.089 0.154 0.244 0.471 0.823 1.249 1.939 O.D. 0.024 0.089 0.154 0.244 0.471 0.823 1.249 1.939

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.