Human PCDH17 ELISA Kit PicoKine®

Protocadherin-17 is a protein that in humans is encoded by the PCDH17 gene. This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The encoded protein contains six extracellular cadherin domains, a transmembrane domain, and a cytoplasmic tail differing from those of the classical cadherins. The encoded protein may play a role in the establishment and function of specific cell-cell connections in the brain.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 24 18 28 23 23 0.94 4.1% Sample 2 32 38 49 35 39 1.58 4.1% Sample 3 342 455 268 298 341 26.85 7.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 24 18 28 23 23 0.94 4.1% Sample 1 24 18 28 23 23 0.94 4.1% Sample 2 32 38 49 35 39 1.58 4.1% Sample 2 32 38 49 35 39 1.58 4.1% Sample 3 342 455 268 298 341 26.85 7.9% Sample 3 342 455 268 298 341 26.85 7.9%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 24 32 342 25 58 413 Standard deviation 0.99 1.97 6.94 1.46 4.15 32.5 CV (%) 4.1% 6.2% 2% 5.9% 7.2% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 24 32 342 25 58 413 Standard deviation 0.99 1.97 6.94 1.46 4.15 32.5 CV (%) 4.1% 6.2% 2% 5.9% 7.2% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 24 32 342 25 58 413 Mean (pg/ml) 24 32 342 25 58 413 Standard deviation 0.99 1.97 6.94 1.46 4.15 32.5 Standard deviation 0.99 1.97 6.94 1.46 4.15 32.5 CV (%) 4.1% 6.2% 2% 5.9% 7.2% 7.9% CV (%) 4.1% 6.2% 2% 5.9% 7.2% 7.9%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.045 0.111 0.162 0.288 0.435 0.732 1.181 1.831 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.045 0.111 0.162 0.288 0.435 0.732 1.181 1.831 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.045 0.111 0.162 0.288 0.435 0.732 1.181 1.831 O.D. 0.045 0.111 0.162 0.288 0.435 0.732 1.181 1.831

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.