Mouse MARCO ELISA Kit PicoKine®

Mouse MARCO ELISA Kit PicoKine® (96 Tests) . Quantitate Mouse MARCO in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 150 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

MARCO gene

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 555 600 343 464 491 21.29 4.3% Sample 2 709 1133 780 1104 932 47.79 5.1% Sample 3 7761 5570 7930 9513 7694 168.49 2.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 555 600 343 464 491 21.29 4.3% Sample 1 555 600 343 464 491 21.29 4.3% Sample 2 709 1133 780 1104 932 47.79 5.1% Sample 2 709 1133 780 1104 932 47.79 5.1% Sample 3 7761 5570 7930 9513 7694 168.49 2.2% Sample 3 7761 5570 7930 9513 7694 168.49 2.2%

Gene Name

Marco

Gene ID

17167

UniProt

Q60754

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q70-S518

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA) .

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 555 709 7761 438 1039 5548 Standard deviation 30.53 44.88 305.01 27.81 41.04 220.81 CV (%) 5.5% 6.3% 3.9% 6.4% 4% 4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 555 709 7761 438 1039 5548 Standard deviation 30.53 44.88 305.01 27.81 41.04 220.81 CV (%) 5.5% 6.3% 3.9% 6.4% 4% 4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 555 709 7761 438 1039 5548 Mean (pg/ml) 555 709 7761 438 1039 5548 Standard deviation 30.53 44.88 305.01 27.81 41.04 220.81 Standard deviation 30.53 44.88 305.01 27.81 41.04 220.81 CV (%) 5.5% 6.3% 3.9% 6.4% 4% 4% CV (%) 5.5% 6.3% 3.9% 6.4% 4% 4%

Sensitivity

<150 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant mouse Marco

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.024 0.065 0.117 0.221 0.452 0.962 1.893 2.668 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.024 0.065 0.117 0.221 0.452 0.962 1.893 2.668 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 Concentration (ng/ml) 0 0.312 0.62 1.25 2.5 5 10 20 O.D. 0.024 0.065 0.117 0.221 0.452 0.962 1.893 2.668 O.D. 0.024 0.065 0.117 0.221 0.452 0.962 1.893 2.668

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.