Human PCPE1 ELISA Kit PicoKine®

Procollagen C-endopeptidase enhancer 1 is an enzyme that in humans is encoded by the PCOLCE gene. Fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and carboxyl-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. This gene encodes a glycoprotein which binds and drives the enzymatic cleavage of type I procollagen and heightens C-proteinase activity.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 952 1166 1130 693 985 50.15 5.1% Sample 2 1335 2149 1773 1456 1668 39.37 2.4% Sample 3 18623 16083 10251 14876 14868 793.96 5.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 952 1166 1130 693 985 50.15 5.1% Sample 1 952 1166 1130 693 985 50.15 5.1% Sample 2 1335 2149 1773 1456 1668 39.37 2.4% Sample 2 1335 2149 1773 1456 1668 39.37 2.4% Sample 3 18623 16083 10251 14876 14868 793.96 5.3% Sample 3 18623 16083 10251 14876 14868 793.96 5.3%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 952 1335 18263 716 1356 10353 Standard deviation 53.03 97.05 1313.11 18.83 58.99 792 CV (%) 5.6% 7.3% 7.2% 2.6% 4.4% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 952 1335 18263 716 1356 10353 Standard deviation 53.03 97.05 1313.11 18.83 58.99 792 CV (%) 5.6% 7.3% 7.2% 2.6% 4.4% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 952 1335 18263 716 1356 10353 Mean (pg/ml) 952 1335 18263 716 1356 10353 Standard deviation 53.03 97.05 1313.11 18.83 58.99 792 Standard deviation 53.03 97.05 1313.11 18.83 58.99 792 CV (%) 5.6% 7.3% 7.2% 2.6% 4.4% 7.7% CV (%) 5.6% 7.3% 7.2% 2.6% 4.4% 7.7%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.009 0.08 0.145 0.266 0.49 0.888 1.687 2.731 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.009 0.08 0.145 0.266 0.49 0.888 1.687 2.731 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.009 0.08 0.145 0.266 0.49 0.888 1.687 2.731 O.D. 0.009 0.08 0.145 0.266 0.49 0.888 1.687 2.731

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.