Human Protogenin ELISA Kit PicoKine®

This gene encodes a member of the immunoglobulin superfamily. The encoded transmembrane protein has been associated with the development of various tissues, especially neurogenesis. It has been suggested that this gene may be associated with attention deficit hyperactivity disorder (ADHD) .

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 26 28 20 24 25 0.68 2.8% Sample 2 62 45 51 47 51 4 7.8% Sample 3 252 350 352 333 322 12.07 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 26 28 20 24 25 0.68 2.8% Sample 1 26 28 20 24 25 0.68 2.8% Sample 2 62 45 51 47 51 4 7.8% Sample 2 62 45 51 47 51 4 7.8% Sample 3 252 350 352 333 322 12.07 3.8% Sample 3 252 350 352 333 322 12.07 3.8%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 24 43 311 26 36 260 Standard deviation 1.47 3.05 14.96 1.73 1.13 12.45 CV (%) 6.1% 7.1% 4.8% 6.6% 3.1% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 24 43 311 26 36 260 Standard deviation 1.47 3.05 14.96 1.73 1.13 12.45 CV (%) 6.1% 7.1% 4.8% 6.6% 3.1% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 24 43 311 26 36 260 Mean (pg/ml) 24 43 311 26 36 260 Standard deviation 1.47 3.05 14.96 1.73 1.13 12.45 Standard deviation 1.47 3.05 14.96 1.73 1.13 12.45 CV (%) 6.1% 7.1% 4.8% 6.6% 3.1% 4.8% CV (%) 6.1% 7.1% 4.8% 6.6% 3.1% 4.8%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.068 0.129 0.191 0.267 0.492 0.92 1.532 2.273 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.068 0.129 0.191 0.267 0.492 0.92 1.532 2.273 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.068 0.129 0.191 0.267 0.492 0.92 1.532 2.273 O.D. 0.068 0.129 0.191 0.267 0.492 0.92 1.532 2.273

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.