Human Protogenin ELISA Kit PicoKine®

Human Protogenin ELISA Kit PicoKine® (96 Tests) . Quantitate Human Protogenin in cell culture supernatants, serum and plasma (heparin, EDTA) . Sensitivity: 8 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

This gene encodes a member of the immunoglobulin superfamily. The encoded transmembrane protein has been associated with the development of various tissues, especially neurogenesis. It has been suggested that this gene may be associated with attention deficit hyperactivity disorder (ADHD) .

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 26 28 20 24 25 0.68 2.8% Sample 2 62 45 51 47 51 4 7.8% Sample 3 252 350 352 333 322 12.07 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 26 28 20 24 25 0.68 2.8% Sample 1 26 28 20 24 25 0.68 2.8% Sample 2 62 45 51 47 51 4 7.8% Sample 2 62 45 51 47 51 4 7.8% Sample 3 252 350 352 333 322 12.07 3.8% Sample 3 252 350 352 333 322 12.07 3.8%

Gene Name

PRTG

Gene ID

283659

UniProt

Q2VWP7

Reactivity

Human

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: F36-A952

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA)

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 24 43 311 26 36 260 Standard deviation 1.47 3.05 14.96 1.73 1.13 12.45 CV (%) 6.1% 7.1% 4.8% 6.6% 3.1% 4.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 24 43 311 26 36 260 Standard deviation 1.47 3.05 14.96 1.73 1.13 12.45 CV (%) 6.1% 7.1% 4.8% 6.6% 3.1% 4.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 24 43 311 26 36 260 Mean (pg/ml) 24 43 311 26 36 260 Standard deviation 1.47 3.05 14.96 1.73 1.13 12.45 Standard deviation 1.47 3.05 14.96 1.73 1.13 12.45 CV (%) 6.1% 7.1% 4.8% 6.6% 3.1% 4.8% CV (%) 6.1% 7.1% 4.8% 6.6% 3.1% 4.8%

Sensitivity

<8 pg/mL

Components

Storage Conditions

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Specificity

Natural and recombinant human PRTG

Anticoagulant

Heparin or EDTA

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.068 0.129 0.191 0.267 0.492 0.92 1.532 2.273 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.068 0.129 0.191 0.267 0.492 0.92 1.532 2.273 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.068 0.129 0.191 0.267 0.492 0.92 1.532 2.273 O.D. 0.068 0.129 0.191 0.267 0.492 0.92 1.532 2.273

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.