Human PREP ELISA Kit PicoKine®

Prolyl endopeptidase (PE) also known as prolyl oligopeptidase or post-proline cleaving enzyme is an enzyme that in humans is encoded by the PREP gene. The protein encoded by this gene is a cytosolic prolyl endopeptidase that cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Prolyl endopeptidases have been reported to be involved in the maturation and degradation of peptide hormones and neuropeptides.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 266 163 194 185 202 13.39 6.6% Sample 2 567 457 337 594 489 20.19 4.1% Sample 3 4581 3565 3862 4005 4003 136.51 3.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 266 163 194 185 202 13.39 6.6% Sample 1 266 163 194 185 202 13.39 6.6% Sample 2 567 457 337 594 489 20.19 4.1% Sample 2 567 457 337 594 489 20.19 4.1% Sample 3 4581 3565 3862 4005 4003 136.51 3.4% Sample 3 4581 3565 3862 4005 4003 136.51 3.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 266 567 4581 259 412 4888 Standard deviation 18.97 26.76 349.53 8.52 14.05 147.62 CV (%) 7.1% 4.7% 7.6% 3.3% 3.4% 3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 266 567 4581 259 412 4888 Standard deviation 18.97 26.76 349.53 8.52 14.05 147.62 CV (%) 7.1% 4.7% 7.6% 3.3% 3.4% 3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 266 567 4581 259 412 4888 Mean (pg/ml) 266 567 4581 259 412 4888 Standard deviation 18.97 26.76 349.53 8.52 14.05 147.62 Standard deviation 18.97 26.76 349.53 8.52 14.05 147.62 CV (%) 7.1% 4.7% 7.6% 3.3% 3.4% 3% CV (%) 7.1% 4.7% 7.6% 3.3% 3.4% 3%

Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x) |100ul Avidin-Biotin-Peroxidase Complex (100x) |100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB) |10ml Stop Solution|10ml Wash Buffer (25x) |20ml Adhesive plate sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.084 0.204 0.313 0.491 0.782 1.221 1.703 2.097 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.084 0.204 0.313 0.491 0.782 1.221 1.703 2.097 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.084 0.204 0.313 0.491 0.782 1.221 1.703 2.097 O.D. 0.084 0.204 0.313 0.491 0.782 1.221 1.703 2.097

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.